Detailed information of TA system TA216572

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 2467417..2468252
• Replicon: chromosome
• Accession: NZ_CP083492
• Organism: Escherichia coli strain elppa8

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: K9U11_RS11865
• Protein ID: WP_042047257.1
• Coordinates: 2467417..2467794 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: S1NM52
• Locus tag: K9U11_RS11870
• Protein ID: WP_001295723.1
• Coordinates: 2467884..2468252 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K9U11_RS11835 (2463602)	2463602..2463796	+	195	WP_071208568.1	Arm DNA-binding domain-containing protein	-
K9U11_RS11845 (2464582)	2464582..2465697	+	1116	WP_071208569.1	site-specific integrase	-
K9U11_RS11850 (2465790)	2465790..2466638	-	849	WP_042047262.1	DUF4942 domain-containing protein	-
K9U11_RS11855 (2466723)	2466723..2466920	-	198	WP_000839267.1	DUF957 domain-containing protein	-
K9U11_RS11860 (2466932)	2466932..2467420	-	489	WP_042047259.1	DUF5983 family protein	-
K9U11_RS11865 (2467417)	2467417..2467794	-	378	WP_042047257.1	TA system toxin CbtA family protein	Toxin
K9U11_RS11870 (2467884)	2467884..2468252	-	369	WP_001295723.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
K9U11_RS11875 (2468415)	2468415..2468636	-	222	WP_228595092.1	DUF987 domain-containing protein	-
K9U11_RS11880 (2468699)	2468699..2469175	-	477	WP_001186774.1	RadC family protein	-
K9U11_RS11885 (2469191)	2469191..2469676	-	486	WP_000849588.1	antirestriction protein	-
K9U11_RS11890 (2469731)	2469731..2470549	-	819	WP_001234620.1	DUF932 domain-containing protein	-
K9U11_RS11895 (2470649)	2470649..2470882	-	234	WP_001119729.1	DUF905 family protein	-
K9U11_RS11900 (2470961)	2470961..2471416	-	456	WP_000581504.1	IrmA family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14211.22 Da
• Isoelectric Point: 7.2652

>T216572 WP_042047257.1 NZ_CP083492:c2467794-2467417 [Escherichia coli]
MKTLPDTHVREVSCCPSPVTIWQTLLTRLLDQHYSLTLNDTPFVDERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITQGKHPEAKQ

Nucleotide

• Length: 378 bp

>T216572 NZ_CP113168:1454129-1454545 [Klebsiella pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATTATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCC
GCGGTGGACGCCACGACCGACATCCGGGTGGCGCTGCACCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGCCACGCCATCGCGGCGGGCGCCGTCCTGGTGACGAACAATGTGAGAGAGTTTGCGCGGGTGCCTGGACTGACACTGG
AAGACTGGGTTATTTAG

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13592.40 Da
• Isoelectric Point: 6.6255

>AT216572 WP_001295723.1 NZ_CP083492:c2468252-2467884 [Escherichia coli]
VSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFRDADAYPLDQAFPLLMKQLKLMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT216572 NZ_CP113168:1453902-1454132 [Klebsiella pneumoniae]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGATCCGGACTTTATGGCGGAACGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Structures

Antitoxin

• AlphaFold DB: S1NM52