Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 2271259..2271877 Replicon chromosome
Accession NZ_CP083492
Organism Escherichia coli strain elppa8

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag K9U11_RS10910 Protein ID WP_001291435.1
Coordinates 2271659..2271877 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag K9U11_RS10905 Protein ID WP_000344800.1
Coordinates 2271259..2271633 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K9U11_RS10895 (2266348) 2266348..2267541 + 1194 WP_228595861.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
K9U11_RS10900 (2267564) 2267564..2270713 + 3150 WP_228595862.1 efflux RND transporter permease AcrB -
K9U11_RS10905 (2271259) 2271259..2271633 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
K9U11_RS10910 (2271659) 2271659..2271877 + 219 WP_001291435.1 HHA domain-containing protein Toxin
K9U11_RS10915 (2272049) 2272049..2272600 + 552 WP_000102564.1 maltose O-acetyltransferase -
K9U11_RS10920 (2272716) 2272716..2273186 + 471 WP_000136192.1 YlaC family protein -
K9U11_RS10925 (2273350) 2273350..2274900 + 1551 WP_001310610.1 cyclic-guanylate-specific phosphodiesterase PdeB -
K9U11_RS10930 (2274942) 2274942..2275295 - 354 WP_228595863.1 DUF1428 family protein -
K9U11_RS10940 (2275674) 2275674..2275985 + 312 WP_000409911.1 MGMT family protein -
K9U11_RS10945 (2276016) 2276016..2276588 - 573 WP_021566079.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T216571 WP_001291435.1 NZ_CP083492:2271659-2271877 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T216571 NZ_CP113168:835994-836404 [Klebsiella pneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTTCACGGCGTGGTGGCGGC
CCTGGTGCTGTTGGTTCCCTGGCCGCTGAGCTATACCCCGATCTGGCTGCTGCTGCTGTCGCTGGTGGTCTTCGACTGCG
TGCGCAGCCAGCGGCGGATCCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCC
GAATGGGAGATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGGCGCGG
ACAGCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGGAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGG
CGCAGGAGTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT216571 WP_000344800.1 NZ_CP083492:2271259-2271633 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT216571 NZ_CP113168:835747-836013 [Klebsiella pneumoniae]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References