Detailed information of TA system TA216570

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 1220124..1220762
• Replicon: chromosome
• Accession: NZ_CP083492
• Organism: Escherichia coli strain elppa8

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A0A6TXU8
• Locus tag: K9U11_RS05760
• Protein ID: WP_001447010.1
• Coordinates: 1220586..1220762 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: K9U11_RS05755
• Protein ID: WP_001270286.1
• Coordinates: 1220124..1220540 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K9U11_RS05735 (1215276)	1215276..1216217	-	942	WP_001251304.1	ABC transporter permease	-
K9U11_RS05740 (1216218)	1216218..1217231	-	1014	WP_000220396.1	ABC transporter ATP-binding protein	-
K9U11_RS05745 (1217249)	1217249..1218394	-	1146	WP_228595699.1	ABC transporter substrate-binding protein	-
K9U11_RS05750 (1218639)	1218639..1220045	-	1407	WP_000760626.1	PLP-dependent aminotransferase family protein	-
K9U11_RS05755 (1220124)	1220124..1220540	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
K9U11_RS05760 (1220586)	1220586..1220762	-	177	WP_001447010.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
K9U11_RS05765 (1220984)	1220984..1221214	+	231	WP_000494244.1	YncJ family protein	-
K9U11_RS05770 (1221306)	1221306..1223267	-	1962	WP_001303492.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
K9U11_RS05775 (1223340)	1223340..1223876	-	537	WP_000429155.1	DNA-binding transcriptional regulator SutR	-
K9U11_RS05780 (1223968)	1223968..1225143	+	1176	WP_267254694.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6779.86 Da
• Isoelectric Point: 11.2298

>T216570 WP_001447010.1 NZ_CP083492:c1220762-1220586 [Escherichia coli]
VKQSEFRRWLESQGVDVANSSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T216570 NZ_CP113168:358556-358903 [Klebsiella pneumoniae]
ATGTGGGATGTGGAAACGACGGATACGTTTGATGCCTGGTTCGAATTACAAAGTAGAGCTTTAAAAGAGGATATGTTGGC
CACGATGCTCATCTTGTCTGAGTTTGCTCCGCAGCTTGGGCGCCCGTATGTTGATACGGTGAAAGATTCAACGTTTCAGA
ATATGAAAGAACTGCGGGTTCAGCATCATGGGCTCCCCATCCGCGCCTTTTTTGCTTTTGATCCTCTGCGGAAAGCTATC
GTTTTATGTGCGGGCAATAAGGATGGCATAAATGAGAAACGTTTTTACAAAGAGATGATTACTCTGGCTGACAGAGAATT
CAGCCAACACCTGACTAAGGAGCGATAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT216570 WP_001270286.1 NZ_CP083492:c1220540-1220124 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT216570 NZ_CP113168:358903-359202 [Klebsiella pneumoniae]
ATGGCCACGCTAAAAGAACTCATGGCGAAACAGAGTCCTGAAAGCCAGCAGCGTATTGCAGCGAAAGCGGCAGAGATACG
CCAGTCTGTCGCTTTAAACTTGCTTCGAGAAGAGTTGCAAATGTCGCAAACAGAGATGGCTGCGGCTATGGGGGTAAAGC
AGCCAACGATCGCTAAAATGGAACAGACTGATAACGACCCTCGGCTTTCGACCCTAAAAAGCTACATTGCGGCATTGGGG
GGAGAACTCAGTATAAATGTCACATTGCCGACTGGAAAAAAAGTCTCGTTTAATTTGTGA

Structures

Toxin

• AlphaFold DB: A0A0A6TXU8