Detailed information of TA system TA216555

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RelE-RHH
• Location: 13875..14422
• Replicon: plasmid unnamed6
• Accession: NZ_CP083487
• Organism: Escherichia coli strain elppa9

Toxin (Protein)

• Gene name: parE
• Uniprot ID: -
• Locus tag: K9U12_RS26480
• Protein ID: WP_244442846.1
• Coordinates: 14144..14422 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: E1QMW3
• Locus tag: K9U12_RS26475
• Protein ID: WP_000079941.1
• Coordinates: 13875..14144 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K9U12_RS26455 (K9U12_26465)	9693..9998	-	306	WP_001255015.1	LysR family transcriptional regulator	-
K9U12_RS26460 (K9U12_26470)	10026..11240	-	1215	WP_000214121.1	chloramphenicol/florfenicol efflux MFS transporter FloR	-
K9U12_RS26465 (K9U12_26475)	11457..12341	-	885	WP_001447541.1	DUF3363 domain-containing protein	-
K9U12_RS26470 (K9U12_26480)	12372..13517	-	1146	Protein_14	IS91-like element ISVsa3 family transposase	-
K9U12_RS26475 (K9U12_26485)	13875..14144	+	270	WP_000079941.1	type II toxin-antitoxin system antitoxin YacA	Antitoxin
K9U12_RS26480 (K9U12_26490)	14144..14422	+	279	WP_244442846.1	type II toxin-antitoxin system toxin YacB	Toxin
K9U12_RS26485 (K9U12_26495)	14462..15310	+	849	WP_021265674.1	3'-5' exonuclease	-
K9U12_RS26490 (K9U12_26500)	15427..15900	+	474	WP_001334658.1	hypothetical protein	-
K9U12_RS26495 (K9U12_26505)	16377..16643	-	267	WP_000955263.1	hypothetical protein	-
K9U12_RS26500 (K9U12_26510)	16643..17137	-	495	WP_001459505.1	hypothetical protein	-
K9U12_RS26505 (K9U12_26515)	17193..17795	-	603	WP_000517695.1	ProQ/FINO family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	sul2 / floR	-	1..33771	33771

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-91)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10761.43 Da
• Isoelectric Point: 9.7617

>T216555 WP_244442846.1 NZ_CP083487:14144-14422 [Escherichia coli]
MEIFWTMLASQDRKRIRKYIAEQNLMAAIELDERIGYSASSLAGQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKVY
ILRVLHTAQKWP

Nucleotide

• Length: 279 bp

>T216555 NZ_CP113165:668502-668849 [Klebsiella pneumoniae]
ATGTGGGATGTGGAAACGACGGATACGTTTGATGCCTGGTTCGAATTACAAAGTAGAGCTTTAAAAGAGGATATGTTGGC
CACGATGCTCATCTTGTCTGAGTTTGCTCCGCAGCTTGGGCGCCCGTATGTTGATACGGTGAAAGATTCAACGTTTCAGA
ATATGAAAGAACTGCGGGTTCAGCATCATGGGCTCCCCATCCGCGCCTTTTTTGCTTTTGATCCTCTGCGGAAAGCTATC
GTTTTATGTGCGGGCAATAAGGATGGCATAAATGAGAAACGTTTTTACAAAGAGATGATTACTCTGGCTGACAGAGAATT
CAGCCAACACCTGACTAAGGAGCGATAA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10252.58 Da
• Isoelectric Point: 7.2057

>AT216555 WP_000079941.1 NZ_CP083487:13875-14144 [Escherichia coli]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVEAGRQQLERGDVLPHDMVESSAAAWRD
EMSRKVAGK

Nucleotide

• Length: 270 bp

>AT216555 NZ_CP113165:668849-669148 [Klebsiella pneumoniae]
ATGGCCACGCTAAAAGAACTCATGGCGAAACAGAGTCCTGAAAGCCAGCAGCGTATTGCAGCGAAAGCGGCAGAGATACG
CCAGTCTGTCGCTTTAAACTTGCTTCGAGAAGAGTTGCAAATGTCGCAAACAGAGATGGCTGCGGCTATGGGGGTAAAGC
AGCCAACGATCGCTAAAATGGAACAGACTGATAACGACCCTCGGCTTTCGACCCTAAAAAGCTACATTGCGGCATTGGGG
GGAGAACTCAGTATAAATGTCACATTGCCGACTGGAAAAAAAGTCTCGTTTAATTTGTGA

Structures

Antitoxin

• AlphaFold DB: A0A629TXC5