Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 4212441..4213095 Replicon chromosome
Accession NZ_CP083481
Organism Escherichia coli strain elppa9

Toxin (Protein)


Gene name cptA Uniprot ID F4T2L4
Locus tag K9U12_RS20455 Protein ID WP_000244765.1
Coordinates 4212688..4213095 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag K9U12_RS20450 Protein ID WP_000354046.1
Coordinates 4212441..4212707 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K9U12_RS20430 (4208529) 4208529..4209962 - 1434 WP_141031856.1 6-phospho-beta-glucosidase BglA -
K9U12_RS20435 (4210007) 4210007..4210318 + 312 WP_001182951.1 N(4)-acetylcytidine aminohydrolase -
K9U12_RS20440 (4210482) 4210482..4211141 + 660 WP_000250274.1 hemolysin III family protein -
K9U12_RS20445 (4211218) 4211218..4212198 - 981 WP_086518704.1 tRNA-modifying protein YgfZ -
K9U12_RS20450 (4212441) 4212441..4212707 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
K9U12_RS20455 (4212688) 4212688..4213095 + 408 WP_000244765.1 protein YgfX Toxin
K9U12_RS20460 (4213135) 4213135..4213656 - 522 WP_001055874.1 flavodoxin FldB -
K9U12_RS20465 (4213768) 4213768..4214664 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
K9U12_RS20470 (4214689) 4214689..4215399 + 711 WP_000715230.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
K9U12_RS20475 (4215405) 4215405..4217138 + 1734 WP_000813190.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16043.95 Da        Isoelectric Point: 11.5202

>T216542 WP_000244765.1 NZ_CP083481:4212688-4213095 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLIPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T216542 NZ_CP113118:c18316-17972 [Levilactobacillus brevis]
ATGGTAAGCCAAGGTGATATATTTTATGTTAACTTTAATCCAAGCCGTGGTCATGAACAGATGAATAAGCGACCAGCCAT
TGCGCTAAGCAATGATCTAGTCTGTCAGACAAGTAACATGACGATTGTGGCTCCAATTAGCAGTACCAAGCGTAATTTTC
CAATGTATCATCGACTAACTAGTAGTCAAACAGTATATGGAAAAGTATTATTAGATCAAACCATAGCCTTAGATTTACGG
GCAAGACATGTCACTGATGAAACTATTGTCGATCATGTATCGCGTGAAGAACTAGAAGAAATAATCACTTTGTACAAATT
ATTGTTTAGTATTGATGACAAATAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT216542 WP_000354046.1 NZ_CP083481:4212441-4212707 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT216542 NZ_CP113118:c18573-18310 [Levilactobacillus brevis]
ATGGAACTTATTAAAGAACAAACACGCTTAGCAAAGTGGGGAAACTCGAAAGCTGCTAGAATTCCTAGCCAAATTATTAA
ACAACTGAAATTAGATGATAACCAAGATATGACAATAACCATTGAAAATGGATCAATTGTTTTAACACCAATAAAAAAGA
ACCCAACCAATATTCACGAACTATTTAAGGATTGGAAAGATGACGGGAAAAGGGATCACGAGCTTGATTGGGGAAAGTCA
GAAGGTAATGAACTTCAATGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A454A7D7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References