Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 2830566..2831063 Replicon chromosome
Accession NZ_CP083481
Organism Escherichia coli strain elppa9

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag K9U12_RS13950 Protein ID WP_001173343.1
Coordinates 2830566..2830739 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag K9U12_RS13955 Protein ID WP_000398619.1
Coordinates 2830767..2831063 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K9U12_RS13940 (2827236) 2827236..2828693 + 1458 WP_000856827.1 dipeptide/tripeptide permease DtpC -
K9U12_RS13945 (2828930) 2828930..2830447 + 1518 WP_000003806.1 lysine--tRNA ligase -
K9U12_RS13950 (2830566) 2830566..2830739 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
K9U12_RS13955 (2830767) 2830767..2831063 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
K9U12_RS13960 (2831292) 2831292..2831564 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
K9U12_RS13965 (2831576) 2831576..2831806 - 231 WP_000373291.1 4Fe-4S mono-cluster protein YjdI -
K9U12_RS13970 (2831987) 2831987..2833618 + 1632 WP_001216477.1 sensor histidine kinase -
K9U12_RS13975 (2833615) 2833615..2834334 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 2785245..2831063 45818


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T216538 WP_001173343.1 NZ_CP083481:c2830739-2830566 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T216538 NZ_CP113115:964287-964466 [Streptococcus pneumoniae]
ATGCCTATGACACAAAAAGAGATGGTTAAACTCCTTACGGCCCATGGTTGGATAAAGACTAGAGGCGGTAAAGGCTCCCA
TATTAAAATGGAGAAGCAAGGGGAAAGACTTATCACAATCCCTCATGGTGAGCTGAATAAGTACACTGAAAGAGGGATAA
GAAAGCAAGCTGGGTTGTAA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT216538 WP_000398619.1 NZ_CP083481:c2831063-2830767 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT216538 NZ_CP113115:964503-964955 [Streptococcus pneumoniae]
ATGTTAGTTACGTATCCAGCTTTATTTTATTATGACGACACAGACGGAACAGAAGCGACTTATTTTGTCCATTTCCCAGA
TTTTGAATACTCAGCTACACAAGGAGAGGGGATTTCTGAGGCTTTGGCTATGGGGTCGGAGTGGCTAGGGATAACTGTTG
CAGATTTGATAGAGAGTGATGGAGAGTTACCTCAGCCATCAGATATCAATAGCTTGTCTTTAATTGATAATGATCCTTTT
AAAGATGATGAAGATTTCGTGTCAACCTATGACCTTGATAAATCTTTTATTTCTATGGTATCTGTTGATGTATCAGAATA
CCTAGGAAGTCAGGAACCCATTAAAAAGACTTTGACCATACCAAAATGGGCAGATAAGTTGGGACGAGAAATGGGACTTA
ACTTTTCTCAGACTTTGACAGACGCTATTGCAGATAAGAAAGTTCAAGCCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References