Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1939928..1940546 Replicon chromosome
Accession NZ_CP083481
Organism Escherichia coli strain elppa9

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag K9U12_RS09610 Protein ID WP_001291435.1
Coordinates 1940328..1940546 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag K9U12_RS09605 Protein ID WP_000344800.1
Coordinates 1939928..1940302 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K9U12_RS09595 (1935017) 1935017..1936210 + 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
K9U12_RS09600 (1936233) 1936233..1939382 + 3150 WP_001132480.1 efflux RND transporter permease AcrB -
K9U12_RS09605 (1939928) 1939928..1940302 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
K9U12_RS09610 (1940328) 1940328..1940546 + 219 WP_001291435.1 HHA domain-containing protein Toxin
K9U12_RS09615 (1940718) 1940718..1941269 + 552 WP_001522034.1 maltose O-acetyltransferase -
K9U12_RS09620 (1941385) 1941385..1941855 + 471 WP_001490888.1 YlaC family protein -
K9U12_RS09625 (1942019) 1942019..1943569 + 1551 WP_001577872.1 cyclic-guanylate-specific phosphodiesterase PdeB -
K9U12_RS09630 (1943611) 1943611..1943964 - 354 WP_000878140.1 DUF1428 domain-containing protein -
K9U12_RS09640 (1944343) 1944343..1944654 + 312 WP_000409911.1 MGMT family protein -
K9U12_RS09645 (1944685) 1944685..1945257 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T216531 WP_001291435.1 NZ_CP083481:1940328-1940546 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T216531 NZ_CP113106:246522-246932 [Pseudomonas aeruginosa]
ATGCTCAAGTTCATGCTCGACACCAACATCTGCATCTTCACCATCAAAAACCGCCCGCAAGAGGTGCGCGAGGCGTTCCT
GCGCCACCACGATCAGATGTGCATCAGCACCGTGACGCTGATGGAGCTGATCTACGGCGCAGAGAAGTCCTCGAACCCCT
CGCGCAATCTTGCAGACGTGGAAGGCTTCGCTGCTCGTCTGGAGGTGCTCAAGTACGACCAGGAAGCTGCCGCCCACACC
GGCCAGCTCCGCGCTGAACTGGCCCGCCTCGGCAAGCAAATCGGCCCCTATGACCAGATGATTGCCGGGCACGCCCGCTC
TCAGGGCCTGATCGTGGTCACCAATAACCGCCGCGAGTTCGACCGCGTACCCGGCTTGAGAGTTGAGGACTGGGTCGTCC
CAATCGTGTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT216531 WP_000344800.1 NZ_CP083481:1939928-1940302 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT216531 NZ_CP113106:246292-246522 [Pseudomonas aeruginosa]
ATGGAACAAGGTTCTGTTTTTCAGAGCAATCGTAGCCAGGCCGTAAGACTGCCCAAGGCAGTCGCGCTTCCCGATGACGT
TAAGCGTGTGGATGTGGTCGTTGTGGGGCGCGCCAGAATCATCACGCCTGCCGGCGAGTCTTGGGATAGCTGGTTCGATG
GCGAGGGCGTTACTGCCGACTTTATGGCAGAGCGTGAACAGCCTGCCGATCAGGAGCGCGAGACTTTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References