Detailed information of TA system TA216530

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-HTH_XRE
• Location: 1518241..1518946
• Replicon: chromosome
• Accession: NZ_CP083481
• Organism: Escherichia coli strain elppa9

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1F406
• Locus tag: K9U12_RS07755
• Protein ID: WP_000539521.1
• Coordinates: 1518241..1518627 (+)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: K9U12_RS07760
• Protein ID: WP_001280945.1
• Coordinates: 1518617..1518946 (+)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K9U12_RS07735 (1514245)	1514245..1514871	+	627	WP_020233670.1	glutathione S-transferase GstB	-
K9U12_RS07740 (1514868)	1514868..1515983	-	1116	WP_000555050.1	aldose sugar dehydrogenase YliI	-
K9U12_RS07745 (1516094)	1516094..1516477	-	384	WP_000497137.1	biofilm formation regulator BssR	-
K9U12_RS07750 (1516690)	1516690..1518015	+	1326	WP_000049367.1	30S ribosomal protein S12 methylthiotransferase RimO	-
K9U12_RS07755 (1518241)	1518241..1518627	+	387	WP_000539521.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
K9U12_RS07760 (1518617)	1518617..1518946	+	330	WP_001280945.1	DNA-binding transcriptional regulator	Antitoxin
K9U12_RS07765 (1519016)	1519016..1520344	-	1329	WP_000086873.1	GGDEF domain-containing protein	-
K9U12_RS07770 (1520352)	1520352..1522700	-	2349	WP_021523051.1	EAL domain-containing protein	-
K9U12_RS07775 (1522878)	1522878..1523789	-	912	WP_001236019.1	glutathione ABC transporter permease GsiD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (1-124)

Antitoxin

• Cro/C1-type helix-turn-helix domain (53-94)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14293.45 Da
• Isoelectric Point: 9.9296

>T216530 WP_000539521.1 NZ_CP083481:1518241-1518627 [Escherichia coli]
MGVYKARRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVIKKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSKSG
LSSKGTKEIEDDELAAYKKMANAFLAFSNKQIEDLIETGFLIEVKNER

Nucleotide

• Length: 387 bp

>T216530 NZ_CP113106:21057-21335 [Pseudomonas aeruginosa]
ATGATTCTGACCTTTCGCTGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTCGCAATGCTTCATGCGGCTACGGAGCTTCGAGACCTGCGCTCTCCACCTGGAAACCGGTTGG
AGCCGTTGCAGGGAAAGCGGGCGGGCCAACATAGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11982.08 Da
• Isoelectric Point: 10.0101

>AT216530 WP_001280945.1 NZ_CP083481:1518617-1518946 [Escherichia coli]
MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAKVKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSK
WERNEIKPSGPALRILNTLAIKGPEVFAK

Nucleotide

• Length: 330 bp

>AT216530 NZ_CP113106:21347-21652 [Pseudomonas aeruginosa]
ATGGCTACCAATGGTATGCGCCCCATCCATCCTGGGGAAATATTGCGCGATGAGTTTCTGATGGAGTTGGATATCTCTCC
AGCTGCTCTAGCACGCGCTTTGAAAGTCTCCGCTCCGACAGTGAACGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTATTCGTTAGCA
ACTGCTTATGCGGCGAATGGCAAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCACGGATAG

Structures

Toxin

• AlphaFold DB: A0A829GCT6