Detailed information of TA system TA216529

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 1228154..1228938
• Replicon: chromosome
• Accession: NZ_CP083481
• Organism: Escherichia coli strain elppa9

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: V0T0H9
• Locus tag: K9U12_RS06325
• Protein ID: WP_000613626.1
• Coordinates: 1228444..1228938 (+)
• Length: 165 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: L4JCW6
• Locus tag: K9U12_RS06320
• Protein ID: WP_001110447.1
• Coordinates: 1228154..1228447 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K9U12_RS06310 (1223304)	1223304..1224263	-	960	WP_000846343.1	23S rRNA pseudouridine(955/2504/2580) synthase RluC	-
K9U12_RS06315 (1224836)	1224836..1228021	+	3186	WP_001522867.1	ribonuclease E	-
K9U12_RS06320 (1228154)	1228154..1228447	+	294	WP_001110447.1	DUF1778 domain-containing protein	Antitoxin
K9U12_RS06325 (1228444)	1228444..1228938	+	495	WP_000613626.1	GNAT family N-acetyltransferase	Toxin
K9U12_RS06330 (1229033)	1229033..1229986	-	954	WP_001212768.1	flagellar hook-associated protein FlgL	-
K9U12_RS06335 (1229998)	1229998..1231641	-	1644	WP_001522865.1	flagellar hook-associated protein FlgK	-
K9U12_RS06340 (1231707)	1231707..1232648	-	942	WP_001522863.1	flagellar assembly peptidoglycan hydrolase FlgJ	-
K9U12_RS06345 (1232648)	1232648..1233745	-	1098	WP_000589326.1	flagellar basal body P-ring protein FlgI	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (81-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-88)

Sequences

Toxin

Protein

• Length: 165 a.a.
• Molecular weight: 18084.00 Da
• Isoelectric Point: 8.2617

>T216529 WP_000613626.1 NZ_CP083481:1228444-1228938 [Escherichia coli]
MIQAPEPLMARHQFTSFCSGVETMDNWLKQRALKNQLAGASRTFVSCDTYSNVLAYYSLASSAVETYVATGRFRRNMPEP
IPVVVLGRLAIDKSLQGQGIGRAMVRDAGLRVLQAAEVIGIRGMLVHALSDQAREFYLRVGFEPSPVDSMILMATLADLQ
ECLK

Nucleotide

• Length: 495 bp

>T216529 NZ_CP113105:c2777636-2777175 [Staphylococcus aureus]
ATGGGATTATATGAAGAAACTTTAATACAACATGATTATATTGAAATAAGAGAGGCTGATGTGCTTCCAGATAATTTAGA
CGGGGTATGGTTAGGAGATTTAATTTTGATAAAGCGTGGTTTATCAGATAGAGAAAAGGCAGGGATTCTCTTTGAAGAAT
TAGCACATAATAAACTTACATACGGTGATATAGCCGATTACTCGAAATTCAACAATCGCAAGTTCGAAAATTACGCAAGA
CGACACGGCTTTATCTCAGCTGTTCCATTACGCGAAATTGTAGAAGCTTATAATTATGGCGTACGTAACTTGTATGAATT
GTCTGAGTATCTACAATTAAGCGAAGAATACATATTAGAAGCAATAGAACAATATAAAAAGATATATGGTATTGGAACTC
ACTATGGCGAGTATTCTATTACATTTGAGCCGTTGAGAGTTTTTAAATATAAGGAAATATAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11109.69 Da
• Isoelectric Point: 10.5423

>AT216529 WP_001110447.1 NZ_CP083481:1228154-1228447 [Escherichia coli]
MPAANNIPTRRESLNLRIKPAERDLIDRAAKARGKNRTEFVLEAARAAAEEALIEQRIFRIDSDDYQLFLNRLDQPPAPN
AALRKTMQTPAPWEQKK

Nucleotide

• Length: 294 bp

>AT216529 NZ_CP113105:c2777963-2777649 [Staphylococcus aureus]
ATGTGCTTTTCAAAAAGAATGAAACAATCAAGAGAAAAACAAGGTATGACTTTGGCCGAACTAGGAAGAAAAATTGGTAA
AACTGAAGCTACTGTACAACGTTATGAAAGCGGAAATATCAAAAATCTAAAAAACGATACTATAGAAAGTATAGCTACTG
CATTAAATGTTAATCCTGCGTATTTAATGGGGTGGGTTGAAGAAAACGATGATGAAGTACAACATCGTGCAGCTCATCTT
GAAGGAGAATTGACAGATGACGAATGGCAAAGAGTTTTAGATTATGCAGATTATATAAGAAGCAAACGTAAGTAA

Structures

Toxin

• AlphaFold DB: V0T0H9

Antitoxin

• AlphaFold DB: L4JCW6