Detailed information of TA system TA216528

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 751838..752476
• Replicon: chromosome
• Accession: NZ_CP083481
• Organism: Escherichia coli strain elppa9

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A0D8WF76
• Locus tag: K9U12_RS03745
• Protein ID: WP_001306887.1
• Coordinates: 752300..752476 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: K9U12_RS03740
• Protein ID: WP_001270286.1
• Coordinates: 751838..752254 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K9U12_RS03720 (746990)	746990..747931	-	942	WP_001251315.1	ABC transporter permease	-
K9U12_RS03725 (747932)	747932..748945	-	1014	WP_001523231.1	ABC transporter ATP-binding protein	-
K9U12_RS03730 (748963)	748963..750108	-	1146	WP_020233879.1	ABC transporter substrate-binding protein	-
K9U12_RS03735 (750353)	750353..751759	-	1407	WP_001523229.1	PLP-dependent aminotransferase family protein	-
K9U12_RS03740 (751838)	751838..752254	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
K9U12_RS03745 (752300)	752300..752476	-	177	WP_001306887.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
K9U12_RS03750 (752698)	752698..752928	+	231	WP_000494241.1	YncJ family protein	-
K9U12_RS03755 (753020)	753020..754981	-	1962	WP_024166512.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
K9U12_RS03760 (755054)	755054..755590	-	537	WP_020233877.1	DNA-binding transcriptional regulator SutR	-
K9U12_RS03765 (755643)	755643..756854	+	1212	WP_023143671.1	BenE family transporter YdcO	-
K9U12_RS03770 (756962)	756962..757420	-	459	WP_000526135.1	IS200/IS605-like element IS200C family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	756962..757420	458

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6768.88 Da
• Isoelectric Point: 11.5336

>T216528 WP_001306887.1 NZ_CP083481:c752476-752300 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFRGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T216528 NZ_CP113105:2695435-2695611 [Staphylococcus aureus]
TTGGATAGATGGTGGCTATCTGAGTATAAGGAGGTGGTGCCTATGGTGGCATTACTGAAATCTTTAGAAAGGAGACGCCT
AATGATTACAATTAGTACCATGTTGCAGTTTGGTTTATTCCTTATTGCATTGATAGGTCTAGTAATCAAGCTTATTGAAT
TAAGCAATAAAAAATAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT216528 WP_001270286.1 NZ_CP083481:c752254-751838 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT216528 NZ_CP113105:c2695773-2695599 [Staphylococcus aureus]
ATTTGATATTTATATTATGGTGTGTTAATTTATATATAGAAAAAGGGCAACATGCGCAAACATGTTACCCTAATGAGCCC
GTTAAAAAGACGGTGGCTATTTTAGATTAAAGATTAAATTAATAACCATTTAACCATCGAAACCAGCCAAAGTTAGCGAT
GGTTATTTTTTATTG

Structures

Toxin

• AlphaFold DB: A0A0D8WF76