Detailed information of TA system TA216527

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 649044..650005
• Replicon: chromosome
• Accession: NZ_CP083481
• Organism: Escherichia coli strain elppa9

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: A0A1Q5ZNB7
• Locus tag: K9U12_RS03300
• Protein ID: WP_021523084.1
• Coordinates: 649310..650005 (+)
• Length: 232 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: K9U12_RS03295
• Protein ID: WP_001296726.1
• Coordinates: 649044..649310 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K9U12_RS03295 (649044)	649044..649310	+	267	WP_001296726.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
K9U12_RS03300 (649310)	649310..650005	+	696	WP_021523084.1	HipA N-terminal domain-containing protein	Toxin
K9U12_RS03305 (650205)	650205..651557	-	1353	WP_001551127.1	SIR2 family protein	-
K9U12_RS03310 (651772)	651772..652804	-	1033	Protein_650	ISNCY family transposase	-
K9U12_RS03315 (653171)	653171..654070	-	900	WP_001523294.1	LysR family transcriptional regulator	-
K9U12_RS03320 (654209)	654209..654517	+	309	WP_001221046.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	651772..652590	818

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (4-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-69)

Sequences

Toxin

Protein

• Length: 232 a.a.
• Molecular weight: 25773.79 Da
• Isoelectric Point: 9.9928

>T216527 WP_021523084.1 NZ_CP083481:649310-650005 [Escherichia coli]
MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLANRYARPLSLSLPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYH
AKSRQPFDLLSEIGRDSVGAVTLIPEDETIQAGGSYRLTPFYDIISAFPVLGGAGIHISDLKLAMGVNASKGKKTAIDKI
YPRHFLATAKVLKFPEVQMHEILSDFARMIPAALDNVKTSLPTDFPENVVTAVETNVLRLHGRLSREYGIK

Nucleotide

• Length: 696 bp

>T216527 NZ_CP113105:2586631-2586993 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAACAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAATTGGATAAAGACTCAGTTATATTATTAGAGCAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACATACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGTTCATCAGAAAAATTAG

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10047.39 Da
• Isoelectric Point: 4.9632

>AT216527 WP_001296726.1 NZ_CP083481:649044-649310 [Escherichia coli]
MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISNFENNPDNTTLTTFFKILQSLELSMTLCDTKNASPES
TEQQDLEW

Nucleotide

• Length: 267 bp

>AT216527 NZ_CP113105:2586464-2586634 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Toxin

• AlphaFold DB: A0A1Q5ZNB7