Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 46361..47004 Replicon plasmid unnamed1
Accession NZ_CP083479
Organism Escherichia coli strain elppa10

Toxin (Protein)


Gene name vagD Uniprot ID B1LRW4
Locus tag K9U13_RS23455 Protein ID WP_001044768.1
Coordinates 46361..46777 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D2WFK3
Locus tag K9U13_RS23460 Protein ID WP_001261287.1
Coordinates 46774..47004 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K9U13_RS23440 (42860) 42860..43450 - 591 WP_000194575.1 hypothetical protein -
K9U13_RS23445 (43450) 43450..43707 - 258 WP_000343085.1 hypothetical protein -
K9U13_RS23450 (44061) 44061..46199 + 2139 WP_000350635.1 AAA family ATPase -
K9U13_RS23455 (46361) 46361..46777 - 417 WP_001044768.1 type II toxin-antitoxin system VapC family toxin Toxin
K9U13_RS23460 (46774) 46774..47004 - 231 WP_001261287.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
K9U13_RS23465 (47312) 47312..48877 + 1566 WP_000741348.1 AAA family ATPase -
K9U13_RS23470 (48934) 48934..49803 - 870 WP_000253407.1 hypothetical protein -
K9U13_RS23475 (49805) 49805..50893 - 1089 WP_000952231.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid aac(3)-IId / blaTEM-1A / sul2 / aph(3'')-Ib / aph(6)-Id / tet(A) / floR / dfrA14 / sul3 / aph(3')-Ia / sitABCD iroE / iroD / iroC / iroB / iucA / iucB / iucC / iucD / iutA 1..149801 149801


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15197.66 Da        Isoelectric Point: 7.7805

>T216509 WP_001044768.1 NZ_CP083479:c46777-46361 [Escherichia coli]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDEFCARLDAILPWDRA
AVDATTKIKVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPDLVLEDWVK

Download         Length: 417 bp

>T216509 NZ_CP113096:c3227597-3227250 [Pseudomonas citronellolis]
ATGTCCAAGGTCGTCATCCGTCTCACCGATACCGCAGAACAAAGCATCGAAGACCAAGTACACCACTTGGCTCAATTCCA
AGGTTCGCAGGCTGCACTCCAATCGGTGCTGAGCCTTTTGGATGAGATTGAAGAGAAGATTTCTCTTACACCCCAAGGTT
ACCCAATCAGCCAGCAGGCAAGCCTTTTGGGGGTGTTGAACTATCGCGAACTCAATACCGGCCCTTATCGCGTCTTTTAC
GAACTACACGAAGAGCAAGGCGAGGTCGCGGTTATCCTGGTCTTACGCCAGAAGCAGAGTGTGGAACAGCAATTGATTCG
CTACTGCTTGGTGGGGCCTATCGGGTGA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8815.90 Da        Isoelectric Point: 4.3775

>AT216509 WP_001261287.1 NZ_CP083479:c47004-46774 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLEYEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT216509 NZ_CP113096:c3227858-3227607 [Pseudomonas citronellolis]
ATGCGAGTCGAGACAATTAGTTATTTGAAACGCCATGCGGCCGACCTGGATTTATCCGAGCCAATGGTCGTCACACAGAA
CGGTGTTCCTGCCTACGTGATTGAGTCATATGCAGAACGAAAGCAACGTGACGAAGCAATTGCGTTGGTGAAGCTGCTTG
CGATTGGCTCTCGTCAATACGCAGAAGGCAAACATCGCTCTGTAGATGATCTGAAAGCTCGTCTTTCCAGGAGGTTCGAT
CAACCAGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A606Q844

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QFC4

References