Detailed information of TA system TA216507

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 4602314..4603151
• Replicon: chromosome
• Accession: NZ_CP083478
• Organism: Escherichia coli strain elppa10

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: Q3Z4X7
• Locus tag: K9U13_RS22235
• Protein ID: WP_000227784.1
• Coordinates: 4602609..4603151 (+)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: K9U13_RS22230
• Protein ID: WP_001297137.1
• Coordinates: 4602314..4602625 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K9U13_RS22205 (4597334)	4597334..4598281	+	948	WP_021548571.1	cytochrome o ubiquinol oxidase subunit II	-
K9U13_RS22210 (4598303)	4598303..4600294	+	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
K9U13_RS22215 (4600284)	4600284..4600898	+	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
K9U13_RS22220 (4600898)	4600898..4601227	+	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
K9U13_RS22225 (4601239)	4601239..4602129	+	891	WP_000971336.1	heme o synthase	-
K9U13_RS22230 (4602314)	4602314..4602625	+	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
K9U13_RS22235 (4602609)	4602609..4603151	+	543	WP_000227784.1	GNAT family N-acetyltransferase	Toxin
K9U13_RS22240 (4603207)	4603207..4604142	-	936	WP_001297127.1	tetratricopeptide repeat protein	-
K9U13_RS22245 (4604550)	4604550..4605914	+	1365	WP_001000978.1	MFS transporter	-
K9U13_RS22250 (4606042)	4606042..4606533	-	492	WP_001138904.1	nucleotide binding protein YajQ	-
K9U13_RS22255 (4606701)	4606701..4607612	+	912	WP_000705847.1	2-dehydropantoate 2-reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19805.02 Da
• Isoelectric Point: 8.3395

>T216507 WP_000227784.1 NZ_CP083478:4602609-4603151 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T216507 NZ_CP113096:c2654427-2653591 [Pseudomonas citronellolis]
ATGAGCACGGCGGCCGCCTGGCAGGCCGACCTGGAATGCGACTTCCCGCAGCGCCTGGCGCGCCACGGCGAGGTCGTCAG
CGCCTGGCGCGAGGCCGGCGCGGCCGACGCCCCGGCGCTGGTGCTGCTGCACGGCATCGGCTCAGGCTCAGCCTCCTGGC
TGCCGCTGGCGCAGTGCCTGAAGGCGCACGCGCGGCTCATCGCCTGGGACGCCCCCGGCTACGGCGAATCCACGCCGCTG
CCCGAAGCTGCGCCGCGGGCGGAAGCCTACATGCAGCGCCTGCGCGAGCTGCTGGACGCGCTGGACGTGCAGCGCTGCGT
GCTGCTGGGGCATTCCCTGGGCGCGCTGACCGCCCTAGCCCTGGCCAACGCCGAGCCGCGGCGCGTCGCCCGCCTGCTGC
TGCTGAGCCCGGCGCGCGGCTACGGCGCGCGCCCGGCCGAAGGCGAGGCGGTGCGCAGCCGGCGCCTGGCCGCTCTGGCC
AGCGAGGGCATCGCCGGCCTCGCCGAACGCCGCGCCCCGGCCATGCTCGGCGCCAGCCCCAGCGCCGAGGCGCTGGCCTG
GGTGCGCTGGAACATGGCGCGGCTCAAGCCCCACGGCTACCGCCAGGCGGTGGAGCTGCTGTGCGGCGACGACCTGCTGC
GCCTGGGCCGCCCGCCGGTGCCCGTCGAGGTGCTGTGCGGCAGCGAGGACGGCATCACTCTGCCGGCCGCCTGCCGCGAA
GTCGCCGCGGCGCTGGCGGCGCCCTTCCAGCTCGTCCCCGGCGCCGGCCACGCCAGCCCCATCGAATGCCCCGAAGCCCT
GGCCGGACACCTCGTCCGCTTCCTCGACAAGGACTGA

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT216507 WP_001297137.1 NZ_CP083478:4602314-4602625 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT216507 NZ_CP113096:c2653586-2652765 [Pseudomonas citronellolis]
ATGAGTACCGACACCCTCACCGAAAACCAGGAACGCTACATGGTTCCGGGGCTGGAACGCGGCCTGACACTGCTGGGCGA
GTTCACCCGCCACGACCGCACCCTCACCGCCCCGGAACTGGCGCGCCGCCTGCAACTGCCGCGCACCACCATCTTCCGCC
TGCTGGCCACCCTGGAGGCCCTGGGCTTCGTCACCCGCAGCGGCAACGAATACCGCCTGGGCATGGCGGTGCTGCGCCTG
GGCTTCGAATACCTGGCCTCGCTGGAACTCACCGAGCTGGGCCAGCCGATCCTGCGCCGCCTGTGCGACGCCATCGGCTA
CTCCTGCAACCTGGTGGTGCGCGACGGGCGCAACATCGTCTACGTGGCGAAGATCTCGCCGCCGTCGATCCTCACCAGCG
CGGTCAACGTCGGCACCCGCCTGCCGGCCCACGCCACCGTGCTCGGGCGCATCCTCCTGGAAGACCTGGACCTCGCCGCG
CTGCGCGAGCTGTACCCGGAGAAGCAGCTGGAGCGCTTCTCCGAGGGCACCCCGCGCAACACCGAGGAGCTTTTCGACCT
GGTGCAGAACGACCGCCTGCGCGGCTATGCGCTGAGCGAAGGCTTCTTCGAGGCGAGCATCTCCACCGTCGCCGCGCCGG
TGCGCGACCACACCGGGCGCGTCTGCGCCGCCATGGGCGTGACCATCCCCGCCACGCGCATCGCCGAGCACCTGCTGGAG
CCGCTGGTGCGCCAGGTGCGCAACAGCGCCGAAGAACTCTCCGGCCTGCTCGGGCACATGCCCAACCCCAACGTCCTGTC
GCTGCAGAGGGTGCATTCATGA

Structures

Toxin

• PDB: 7BYY

Antitoxin

• AlphaFold DB: A0A0E0Y6B3