Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4568237..4568855 Replicon chromosome
Accession NZ_CP083478
Organism Escherichia coli strain elppa10

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag K9U13_RS22065 Protein ID WP_001291435.1
Coordinates 4568637..4568855 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag K9U13_RS22060 Protein ID WP_000344800.1
Coordinates 4568237..4568611 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K9U13_RS22050 (4563326) 4563326..4564519 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
K9U13_RS22055 (4564542) 4564542..4567691 + 3150 WP_001132469.1 efflux RND transporter permease AcrB -
K9U13_RS22060 (4568237) 4568237..4568611 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
K9U13_RS22065 (4568637) 4568637..4568855 + 219 WP_001291435.1 HHA domain-containing protein Toxin
K9U13_RS22070 (4569027) 4569027..4569578 + 552 WP_000102568.1 maltose O-acetyltransferase -
K9U13_RS22075 (4569694) 4569694..4570164 + 471 WP_000136192.1 YlaC family protein -
K9U13_RS22080 (4570328) 4570328..4571878 + 1551 WP_001299455.1 cyclic-guanylate-specific phosphodiesterase PdeB -
K9U13_RS22085 (4571920) 4571920..4572273 - 354 WP_000878140.1 DUF1428 domain-containing protein -
K9U13_RS22095 (4572652) 4572652..4572963 + 312 WP_000409911.1 MGMT family protein -
K9U13_RS22100 (4572994) 4572994..4573566 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T216506 WP_001291435.1 NZ_CP083478:4568637-4568855 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T216506 NZ_CP113096:2498627-2498809 [Pseudomonas citronellolis]
ATGCGCAGTCGAGAAATCATGGAGTTGATCCGGGCGGATGGCTGGTTCCTTGTGGAAGTGAAAGGCAGTCATCACCAGTT
CCGGCATTTCACCAAGAAGGGGCGGGTGACGGTTCCCCACCCCAGGAGCAACCTGCCGATCGGGACCGTGAACAGCATTC
TGCGCCAGGCCGGCCTGCGATGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT216506 WP_000344800.1 NZ_CP083478:4568237-4568611 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT216506 NZ_CP113096:2498842-2499246 [Pseudomonas citronellolis]
ATGAAATTCCCCGTGGTTCTGCACAAGGACGCCGACTCGGATTACGGCGTGACCATCCCTGACGTGCCTGGCTGCTTCTC
CGCCGGCGGCACCGTCAGCCAGGCGCTGGAAAACGTCCAGGAAGCCCTGGCCCTGCACTTCGAAGGCCTGGTGGCCGACG
GCGAGACGCTCCCGCAAGCCCAGGAAGTGGACACCCACATGGGCAACCCCGACTACGCCGGCGGCGTCTGGGCGGTGGTC
GACTTCGACGTCACCCCCTACCTGGGCAAGGCCGTGCGCTTCAACGCCAGCCTCCCGGAAAACCTCCTGCAACGCATCGA
CGAACGCGTGAAGCGCGACCATCGCTACGCCTCTCGCTCCGGCTTCCTGGCTACCGCCGCGCTGCGTGAGTTGTCTTCGA
GCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References