Detailed information of TA system TA216504

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 3523084..3523722
• Replicon: chromosome
• Accession: NZ_CP083478
• Organism: Escherichia coli strain elppa10

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: K9U13_RS16900
• Protein ID: WP_000813794.1
• Coordinates: 3523546..3523722 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: K9U13_RS16895
• Protein ID: WP_001270286.1
• Coordinates: 3523084..3523500 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K9U13_RS16875 (3519448)	3519448..3519816	-	369	Protein_3280	ABC transporter ATP-binding protein	-
K9U13_RS16880 (3519834)	3519834..3520835	-	1002	Protein_3281	ABC transporter substrate-binding protein	-
K9U13_RS16885 (3520893)	3520893..3521590	+	698	WP_223429336.1	IS1 family transposase	-
K9U13_RS16890 (3521584)	3521584..3523005	-	1422	WP_073511501.1	PLP-dependent aminotransferase family protein	-
K9U13_RS16895 (3523084)	3523084..3523500	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
K9U13_RS16900 (3523546)	3523546..3523722	-	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
K9U13_RS16905 (3523944)	3523944..3524174	+	231	WP_000494244.1	YncJ family protein	-
K9U13_RS16910 (3524266)	3524266..3526227	-	1962	WP_001307191.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
K9U13_RS16915 (3526300)	3526300..3526836	-	537	WP_000429141.1	DNA-binding transcriptional regulator SutR	-
K9U13_RS16920 (3526928)	3526928..3528103	+	1176	WP_267254963.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	3516300..3538860	22560
	-	flank	IS/Tn	-	-	3528143..3529408	1265

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T216504 WP_000813794.1 NZ_CP083478:c3523722-3523546 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T216504 NZ_CP113096:c1967361-1967080 [Pseudomonas citronellolis]
ATGAGCCTGCAGTGGACGCACAAGGCGGCGGCCGACCTGGACGGCCTGTACGACCACTACGTGGTGCTGATCGGCCCGGA
GAAGGCGCTGAAGGCCATCCAGGACGTGGTCGGCCAGGTGAAGGCGCTGGCCGACCTCAGCCTGAGCAGCGTCGGCCGGC
CCAGCGAGGTGCCCGGCGTGCGCGAATTGCCGCTGGAACGCTGGCCCTACCAGGCCGCCTACCGGGTAAAAGGCCGCGAT
GTACAAATTTTGCGCATCGATTCTGTGGATAACCCGGGGTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT216504 WP_001270286.1 NZ_CP083478:c3523500-3523084 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT216504 NZ_CP113096:c1967585-1967358 [Pseudomonas citronellolis]
TTGACCACCGTAATCTCCTTCCGCGCCGATGACGCCCTGGCCAAGGCCCTGGACCAGCTGGCCCGGGCCACCCACCGGGA
CCGCCCCTACCACCTGCGCCAGGCCCTGGCTCAGTACCTGGAGCGCCAGAGCTGGCACGTGGCGGCCATCGACGAGGGCC
TGGCCGACGCCAACGCCGGGCACCTGCTGCCGCACGCCGAGATAGAAGCCAAGTGGGGGCTGGCATGA

Structures

Toxin

• PDB: 6HPB