Detailed information of TA system TA216503

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 3426521..3428109
• Replicon: chromosome
• Accession: NZ_CP083478
• Organism: Escherichia coli strain elppa10

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: U9XU38
• Locus tag: K9U13_RS16490
• Protein ID: WP_001125455.1
• Coordinates: 3426787..3428109 (+)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: K9U13_RS16485
• Protein ID: WP_001296726.1
• Coordinates: 3426521..3426787 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K9U13_RS16485 (3426521)	3426521..3426787	+	267	WP_001296726.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
K9U13_RS16490 (3426787)	3426787..3428109	+	1323	WP_001125455.1	type II toxin-antitoxin system serine/threonine protein kinase toxin HipA	Toxin
K9U13_RS16495 (3428421)	3428421..3428629	+	209	Protein_3204	AraC family transcriptional regulator	-
K9U13_RS16500 (3429063)	3429063..3429626	+	564	WP_001296758.1	type 1 fimbrial major subunit FimA	-
K9U13_RS16505 (3429987)	3429987..3430697	+	711	WP_001195166.1	fimbria/pilus periplasmic chaperone	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	fimA	3426521..3442610	16089

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (149-401)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-69)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49321.93 Da
• Isoelectric Point: 8.8753

>T216503 WP_001125455.1 NZ_CP083478:3426787-3428109 [Escherichia coli]
MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLASRYARPLSLSLPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYH
AKSRQPFDLLSEIGRDSVGAVTLLPENETITRPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVPDAEIIKAGNVRALAVERFDRRW
NAERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIARIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQ
AGGSYRLTPFYDIISAFPVLGGTGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLRFPEVQMHEILSDFARMIP
AALDNVKTSLPTDFPENVVTAVETNVLRLHGRLSREYGSK

Nucleotide

• Length: 1323 bp

>T216503 NZ_CP113096:c982272-981907 [Pseudomonas citronellolis]
ATGGTGAAGGCGCTGTTCGACACCAACGTCCTCATCGACTACCTCAACGGCCACGAACAGGCCCGCGACGAGTTGCGCCG
CTACGATGACCCCGCCATCAGCATCGTCACCTGGATGGAGGTGATGATCGGCGCCACGCCCGCCACCGCCGCGGCCACCC
GCGCCTTCCTCGACAGCTTCGCCCTGGTGCCGCTCGACGCGTCCGTCGCCGAGCGCGCCGTGGCGGTGCGCCAGGCGCTG
CGGGTGAAGCTGCCGGACGCCATCATCAAGGCCAGCGCCGAGGTGCAGGGGCGCCTGCTGGTCACCCGCAACACCAGGGA
CTTCCCCGTCGACGACGCCGGCGTGCGCCTGCCCTACCGGCTCTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10047.39 Da
• Isoelectric Point: 4.9632

>AT216503 WP_001296726.1 NZ_CP083478:3426521-3426787 [Escherichia coli]
MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISNFENNPDNTTLTTFFKILQSLELSMTLCDTKNASPES
TEQQDLEW

Nucleotide

• Length: 267 bp

>AT216503 NZ_CP113096:c982481-982266 [Pseudomonas citronellolis]
ATGCGTACCCTGGTCGACATCCCTGCCGAACATCTCGAAGCGCTCAATGCCCTGGGCGAGCAACAGCAGCAATCGCGCGC
CGCGCTGATCCGCGAGGCGGTCGCCGAGTACCTGGTCCGGCACCGCCGGCAGGATGCCGAGGCCGCCTTCGGCCTGTGGA
ACGGCAGCGCCGGCGACGGCCTGGCCTACCAGGAGAAGGTGCGCGAGGAATGGTGA

Structures

Toxin

• AlphaFold DB: U9XU38