Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 2922045..2922876 Replicon chromosome
Accession NZ_CP083478
Organism Escherichia coli strain elppa10

Toxin (Protein)


Gene name yeeV Uniprot ID S1P968
Locus tag K9U13_RS14020 Protein ID WP_000854814.1
Coordinates 2922045..2922419 (-) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID B3Y195
Locus tag K9U13_RS14025 Protein ID WP_001285585.1
Coordinates 2922508..2922876 (-) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K9U13_RS13980 (2917442) 2917442..2918608 + 1167 WP_073511510.1 serine-type D-Ala-D-Ala carboxypeptidase DacD -
K9U13_RS13985 (2918727) 2918727..2919200 + 474 WP_001105408.1 DNA gyrase inhibitor SbmC -
K9U13_RS13990 (2919397) 2919397..2920455 + 1059 WP_073511509.1 FUSC family protein -
K9U13_RS13995 (2920627) 2920627..2920956 + 330 WP_000450409.1 DUF496 family protein -
K9U13_RS14000 (2921057) 2921057..2921191 - 135 WP_001297944.1 EutP/PduV family microcompartment system protein -
K9U13_RS14005 (2921311) 2921311..2921439 + 129 Protein_2717 transposase domain-containing protein -
K9U13_RS14010 (2921728) 2921728..2921808 - 81 Protein_2718 hypothetical protein -
K9U13_RS14015 (2921854) 2921854..2922048 - 195 WP_000988600.1 DUF5983 family protein -
K9U13_RS14020 (2922045) 2922045..2922419 - 375 WP_000854814.1 type IV toxin-antitoxin system cytoskeleton-binding toxin CbtA Toxin
K9U13_RS14025 (2922508) 2922508..2922876 - 369 WP_001285585.1 type IV toxin-antitoxin system cytoskeleton bundling-enhancing antitoxin CbeA Antitoxin
K9U13_RS14030 (2922950) 2922950..2923171 - 222 WP_000692323.1 DUF987 domain-containing protein -
K9U13_RS14035 (2923234) 2923234..2923710 - 477 WP_001186726.1 RadC family protein -
K9U13_RS14040 (2923726) 2923726..2924205 - 480 WP_000860087.1 antirestriction protein -
K9U13_RS14045 (2924287) 2924287..2925105 - 819 WP_001234629.1 DUF932 domain-containing protein -
K9U13_RS14050 (2925205) 2925205..2925438 - 234 WP_001119729.1 DUF905 family protein -
K9U13_RS14055 (2925517) 2925517..2925972 - 456 WP_000581504.1 IrmA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13898.94 Da        Isoelectric Point: 7.8522

>T216494 WP_000854814.1 NZ_CP083478:c2922419-2922045 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Download         Length: 375 bp

>T216494 NZ_CP113085:3867082-3867297 [Pantoea agglomerans]
ATGAACAAAACCCTGACTAAAACCGATTATTTGATGCGACTGCGGCGCTGCCGCTCTCTTGATACCCTGGAAAGGGTGAT
TGAAAAAAATAAGTATGAATTACCGGAAGATGAATTAGCGGTATTTTACTCAGCAGCTGACCATCGCCTGGCGGAACTGA
CGATGAACAAGCTCTACGATAAAGTCCCGGGTTCAGTATGGAAATTTGTCCGTTAA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13593.48 Da        Isoelectric Point: 6.3139

>AT216494 WP_001285585.1 NZ_CP083478:c2922876-2922508 [Escherichia coli]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLSSCGYVYLAVYPTPEMKN

Download         Length: 369 bp

>AT216494 NZ_CP113085:3866680-3867057 [Pantoea agglomerans]
ATGGACGAATACTCACCGAAACGGCATGATATTGCCCAGCTTAAATACCTTTGTGAGAGTCTGTTTGACGACAGCATGGC
AACATTAACCGACAGTCATCATGGCTGGGTTAATGATCCCACTTCTGAAAGCAATCTACAGCTTAACGATTTGATTGAGC
ATATCGCCTCTTTCACCATGAATTACAAAATTAAGCACGTTGAAGATGAAGCGCTGATTTCGCAAATCGATGAATATCTT
GATGATACTTTTATGCTATTCAGCAATTATGGTGTCAATAGTCCGGATCTTCAGCGGTGGCAACGTTCAGCAAAACGCTT
ATTTAATCTGTTCACTGAAGAGTGCGCTTTTCTCCAGCAACCGAGCCATTCATTATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FY50

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LW60

References