Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 2034129..2034712 Replicon chromosome
Accession NZ_CP083478
Organism Escherichia coli strain elppa10

Toxin (Protein)


Gene name mazF Uniprot ID U9XFN8
Locus tag K9U13_RS09625 Protein ID WP_000254745.1
Coordinates 2034377..2034712 (+) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S1P3W5
Locus tag K9U13_RS09620 Protein ID WP_000581937.1
Coordinates 2034129..2034377 (+) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K9U13_RS09610 (2030468) 2030468..2031769 + 1302 WP_000046790.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -
K9U13_RS09615 (2031817) 2031817..2034051 + 2235 WP_000226815.1 GTP pyrophosphokinase -
K9U13_RS09620 (2034129) 2034129..2034377 + 249 WP_000581937.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
K9U13_RS09625 (2034377) 2034377..2034712 + 336 WP_000254745.1 endoribonuclease MazF Toxin
K9U13_RS09630 (2034783) 2034783..2035574 + 792 WP_001071648.1 nucleoside triphosphate pyrophosphohydrolase -
K9U13_RS09635 (2035802) 2035802..2037439 + 1638 WP_000210878.1 CTP synthase (glutamine hydrolyzing) -
K9U13_RS09640 (2037527) 2037527..2038825 + 1299 WP_000036723.1 phosphopyruvate hydratase -
K9U13_RS09645 (2038881) 2038881..2039243 - 363 WP_000034929.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-109)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12158.08 Da        Isoelectric Point: 8.4777

>T216491 WP_000254745.1 NZ_CP083478:2034377-2034712 [Escherichia coli]
MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPYTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

>T216491 NZ_CP113085:2356688-2357041 [Pantoea agglomerans]
GTGTGGATGATTAAAACGACGGAGAGGTTTGATCGCTGGTTTACCTTGCTTAATGAGAGCGATCGCGCCTGCGTACTGGC
AGCATTAATGGTGTTGCGCGAAAAGGGGCCGGGGTTGTCCAGGCCATACGCGGATACGCTAAAAGGCTCTGTTTATATCA
ACATGAAAGAGCTGCGTATTCAGAGTCGCGGCGATCCGATAAGGGCTTTCTTTGCTTTTGACCCAAACCGAACGGCGATT
GTGCTTTGTGCCGGAAATAAGGTGGGCAATGAAAAGCGTTTTTATCAGGAGATGCTTCCGGTTGCCGATCGGGAATTTAC
ACACTGGCTGAATAGTTTTAAAGATGAGGAGTAA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9355.74 Da        Isoelectric Point: 4.4665

>AT216491 WP_000581937.1 NZ_CP083478:2034129-2034377 [Escherichia coli]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp

>AT216491 NZ_CP113085:2357046-2357348 [Pantoea agglomerans]
ATGGGCAGAACGCTTGAACAGCTTATTGCGGATGAAAAACCGGAAGTCGTTGCCGACGCACAGGCGATGGCGACGTATAT
CCTGCTCAACATTCACCTTGCCGAGCTGCGCGAGAAAGTGCAGAAAACACAGGTCGAAATGGCAAAGGCGCTGGGCATTA
CACAGCCTACCGTGGCGGGCATGGAGAAGCCGGGCCGCGACCTGAAGCTCTCTACGCTCAAGCGCTATATCGATGCGGCA
GGGGGTAAGCTGCGGCTGGTGGTGGAACTGCCGGACGGCTCGCACTACGGGTTTGAGGTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYM3

Antitoxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CQY
PDB 1MVF
PDB 2MRN
PDB 2MRU
AlphaFold DB A0A7U9LMB4

References