Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 1897795..1898449 Replicon chromosome
Accession NZ_CP083478
Organism Escherichia coli strain elppa10

Toxin (Protein)


Gene name cptA Uniprot ID F4NJ21
Locus tag K9U13_RS09010 Protein ID WP_000244772.1
Coordinates 1898042..1898449 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag K9U13_RS09005 Protein ID WP_000354046.1
Coordinates 1897795..1898061 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K9U13_RS08980 (1893083) 1893083..1893826 + 744 WP_000951964.1 SDR family oxidoreductase -
K9U13_RS08985 (1893883) 1893883..1895316 - 1434 WP_001363803.1 6-phospho-beta-glucosidase BglA -
K9U13_RS08990 (1895361) 1895361..1895672 + 312 WP_001182944.1 N(4)-acetylcytidine aminohydrolase -
K9U13_RS08995 (1895836) 1895836..1896495 + 660 WP_000250274.1 hemolysin III family protein -
K9U13_RS09000 (1896572) 1896572..1897552 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
K9U13_RS09005 (1897795) 1897795..1898061 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
K9U13_RS09010 (1898042) 1898042..1898449 + 408 WP_000244772.1 protein YgfX Toxin
K9U13_RS09015 (1898489) 1898489..1899010 - 522 WP_001055874.1 flavodoxin FldB -
K9U13_RS09020 (1899122) 1899122..1900018 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
K9U13_RS09025 (1900043) 1900043..1900753 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
K9U13_RS09030 (1900759) 1900759..1902492 + 1734 WP_000813215.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16048.02 Da        Isoelectric Point: 11.2511

>T216490 WP_000244772.1 NZ_CP083478:1898042-1898449 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWCIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T216490 NZ_CP113085:507158-507511 [Pantoea agglomerans]
ATGTGGACCGTACTCCTGAGCCCGCGCTTTGAAAGCTGGCTAAGTGAGCAGGAAGAAGCCCTGCAGGAAAAAATGTTAGC
GGATCTGGGAAAGTTAAAAGTATACGGCCCTGATTTGCCTCGCCCTTATGCTGATACTCTCAAGGGCTCGCAGTACAGGA
ATATGAAAGAACTCCGCGTACAGTTTTCAGGAAAGCCAATTCGGGCATTTTATGCATTTGACCCTGTCCGCCAGGCTATT
GTGTTATGTGCCGGAGATAAGAGTCATGACAAACGTTTTTATATCCGAATGATACGCATCGCTGACGATGAATTTTCAGC
CTGGCTGGCTGAACAGGAGAAGGGAAATAATTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT216490 WP_000354046.1 NZ_CP083478:1897795-1898061 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT216490 NZ_CP113085:507511-507810 [Pantoea agglomerans]
ATGAGAACCCTGGATGATGTTATCGCCGACTTCTCCCCTGAGAGTCAGGCGCGAATCAAAAAAATGTCTGATGAAATGAT
TCTGGAAGTCGGGCTTCAGATGATTCGCGAAGAGCTTCAGTTATCACAAAAGACGCTCGCCGAGGCCATGGGCGTGCGTC
AGCCTGCCGTTGCTCAGATTGAGCAACGCGGAAATGAGATTAAGGTTGCCACGCTGAAACGCTATGTCGAAGCAATGGGC
GGAAAACTCAGTCTTGATGTGGAACTTCCGACCGGAAAAAGAGTGGCCTTTCGCATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYB4

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References