Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 541173..541670 Replicon chromosome
Accession NZ_CP083478
Organism Escherichia coli strain elppa10

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag K9U13_RS02530 Protein ID WP_001173343.1
Coordinates 541173..541346 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag K9U13_RS02535 Protein ID WP_000398619.1
Coordinates 541374..541670 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K9U13_RS02520 (537843) 537843..539300 + 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -
K9U13_RS02525 (539537) 539537..541054 + 1518 WP_001295074.1 lysine--tRNA ligase -
K9U13_RS02530 (541173) 541173..541346 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
K9U13_RS02535 (541374) 541374..541670 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
K9U13_RS02540 (541897) 541897..542169 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
K9U13_RS02545 (542181) 542181..542411 - 231 WP_000371698.1 4Fe-4S mono-cluster protein YjdI -
K9U13_RS02550 (542592) 542592..544223 + 1632 WP_001216461.1 sensor histidine kinase -
K9U13_RS02555 (544220) 544220..544939 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T216485 WP_001173343.1 NZ_CP083478:c541346-541173 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T216485 NZ_CP113083:c2243219-2242809 [Rhodopseudomonas palustris]
ATGAGTGGCTGGATGCTCGATACCAACGTCGCCAGCCATGTCATCAGGGGCGACCGGCGCGAGATCATCGAGCGGCTAGT
CGCTTTGCCGATCTCGGACGTCGTCATCTCATCGATCACAGAAGGTGAACTCCTCTACGGCCTGGCTAAGCGCGGCTATC
CCACCGCGTTATCCGAACGCGTCCGAGAATTTCTACTCCGCGTCGACGTCCTACCGTGGGATCACGAGGTGACGAAGACT
TACGCCGACCTGCGCGCAGTCTGCGAAGCCAAAGGCGTCACACTCTCTCCTCTCGACATGATGATCGCTGCCCATGCCGC
TGCGACAAACGCAACGTTGGTCACGCGCGACAAGGCCTTCAGCCGCGTGCCTTCACCATTGCGGATCGAGGACTGGGCAG
AGATGAGTTGA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT216485 WP_000398619.1 NZ_CP083478:c541670-541374 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT216485 NZ_CP113083:c2243479-2243216 [Rhodopseudomonas palustris]
GTGACGTCTCGAACCGCCAAGATTTTCACAACTGGACGCAGCCAAGCTGTCCGACTGCCAGCGGAGTTTCGCTTCGAGGG
CAGCGAGGTGTTTGTACGCCGTGATCCGCGGACCGGCGATGTCATTCTGTCCCGAAAACCGGACTCCTGGGACGGCCTAC
TCCAGCTTCACCAGTCGGCGGATGTACCCGACGACTTCTTGGGACCGTCCGACCGTAGCCAGCTTCCGCAGGATCGCGAT
CCGTTTGAAGGCTGGACCGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References