Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) omega-epsilon-zeta/zeta-epsilon
Location 31843..32980 Replicon plasmid pHJP554-2
Accession NZ_CP083475
Organism Enterococcus faecium strain HJP554

Toxin (Protein)


Gene name zeta Uniprot ID Q54944
Locus tag LA367_RS15320 Protein ID WP_001284311.1
Coordinates 31843..32706 (-) Length 288 a.a.

Antitoxin (Protein)


Gene name epsilon Uniprot ID R2XCR7
Locus tag LA367_RS15325 Protein ID WP_000301765.1
Coordinates 32708..32980 (-) Length 91 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LA367_RS15295 27415..28710 - 1296 WP_002297218.1 ISL3-like element ISEfa11 family transposase -
LA367_RS15300 28955..29206 + 252 Protein_33 GNAT family N-acetyltransferase -
LA367_RS15305 29299..30093 + 795 WP_001096887.1 aminoglycoside O-phosphotransferase APH(3')-IIIa -
LA367_RS15310 30635..30718 + 84 WP_031929417.1 23S rRNA methyltransferase attenuator leader peptide ErmL -
LA367_RS15315 30843..31580 + 738 WP_001038796.1 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B) -
LA367_RS15320 31843..32706 - 864 WP_001284311.1 zeta toxin family protein Toxin
LA367_RS15325 32708..32980 - 273 WP_000301765.1 antitoxin Antitoxin
LA367_RS15330 32997..33212 - 216 WP_001835296.1 peptide-binding protein -
LA367_RS15335 33304..33561 - 258 Protein_40 chromosome partitioning protein ParA -
LA367_RS15340 33617..34297 - 681 WP_001015311.1 IS6-like element IS1216 family transposase -
LA367_RS15345 34829..35929 + 1101 WP_224198038.1 cysteine protease staphopain -
LA367_RS15350 35902..36345 + 444 WP_002318191.1 hypothetical protein -
LA367_RS15355 36656..37036 + 381 WP_010782660.1 MobC family plasmid mobilization relaxosome protein -
LA367_RS15360 37018..37932 + 915 WP_010782659.1 relaxase/mobilization nuclease domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid VanHAX / aph(3')-III / erm(B) - 1..42864 42864
- inside IScluster/Tn aph(3')-III / erm(B) - 22463..34297 11834


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Zeta toxin domain

(27-214)

Antitoxin

Bacterial epsilon antitoxin

(2-90)


Sequences

Toxin        


Download         Length: 288 a.a.        Molecular weight: 32404.02 Da        Isoelectric Point: 6.9964

>T216483 WP_001284311.1 NZ_CP083475:c32706-31843 [Enterococcus faecium]
MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGSGKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELV
KLYEKDVVKHVTPYSNRMTEAIISRLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVMAVPKINSYLGTIERYE
TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETPSISPKETLEKELNRKVSGKEIQ
PTLERIEQKMVLNKHQETPEFKAIQQKLESLQPPTPPIPKTPKLPGI

Download         Length: 864 bp

>T216483 NZ_CP113080:449865-450254 [Acinetobacter baumannii]
TTGCTCAACCATTTGAATTTTAAGCTGAAATATAGTCGTTTTTCGATTATATTTCAGTTTTTTATTGGCTTAAGCTTAGC
AATTCTATTTTATCAATTAATGCCGTTACTATGGTGGTTAGTTGTAATCAGCTTACTATTTATAAGCTTTATTTTCTTTT
TAAAGCGTCCGCAACTTGCAAAAATTGCCTATTTAGATAAAAAACTATGGTCGCTTGCTTATCACTCACAGAACACCATT
TCTCGCGTCAAAATTTTAAAAATAATTGATTATCAGATTTTCATAGTGATCTATTTTGAAGGTCATAAAACACTAACTTC
TATCATTTGGTTTGATCAAATGTCATTAGCAGAGTGGAAAAAGCTTAAAACTTTAGAAAAACTCTATTAA

Antitoxin


Download         Length: 91 a.a.        Molecular weight: 10721.25 Da        Isoelectric Point: 6.4676

>AT216483 WP_000301765.1 NZ_CP083475:c32980-32708 [Enterococcus faecium]
MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEVNLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYS
KQVLNKEKVA

Download         Length: 273 bp

>AT216483 NZ_CP113080:449601-449858 [Acinetobacter baumannii]
ATGTCTGAAGAAATGACCTTGGAAGAACGTAAAGTAATCTATCGCGCACGTCGTGGTTTAAAGGAAATTGATGTCTATTT
TGACCCTTATGTGAAAAATTATTATTTAAAGGCAGACCCGGCAGAAAAAGCGTTGTTTGCTGAGTTGGTAGAACAGGAAG
ATCCTGATTTACTCGATTGGTTTATGGAAGTATCAGAACCACCACGTACCGAATTACGTGACTTTATCTATAAATTAAAA
CACTATGTTCATGGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 1GVN
PDB 3Q8X

Antitoxin

Source ID Structure
PDB 3Q8X
PDB 1GVN
AlphaFold DB A0A829F0A3

References