Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907(toxin)
Location 489193..489764 Replicon chromosome
Accession NZ_CP083473
Organism Enterococcus faecium strain HJP554

Toxin (Protein)


Gene name mazF Uniprot ID Q3Y2B8
Locus tag LA367_RS02295 Protein ID WP_002286801.1
Coordinates 489423..489764 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A828ZZN9
Locus tag LA367_RS02290 Protein ID WP_002323011.1
Coordinates 489193..489423 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LA367_RS02265 484326..485657 + 1332 WP_002286818.1 FAD-containing oxidoreductase -
LA367_RS02270 485679..486305 + 627 WP_002286816.1 cysteine hydrolase -
LA367_RS02275 486488..487069 + 582 WP_002286813.1 TetR/AcrR family transcriptional regulator -
LA367_RS02280 487801..488363 + 563 Protein_452 SOS response-associated peptidase -
LA367_RS02285 488568..488906 - 339 WP_002286804.1 hypothetical protein -
LA367_RS02290 489193..489423 + 231 WP_002323011.1 hypothetical protein Antitoxin
LA367_RS02295 489423..489764 + 342 WP_002286801.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
LA367_RS02300 490614..490799 + 186 WP_002304207.1 hypothetical protein -
LA367_RS02305 491304..491498 + 195 WP_002297028.1 hypothetical protein -
LA367_RS02310 491689..491938 + 250 Protein_458 transposase -
LA367_RS02315 492182..493012 - 831 WP_002286789.1 manganese catalase family protein -
LA367_RS02320 493220..494554 + 1335 WP_002286788.1 ABC transporter substrate-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13302.68 Da        Isoelectric Point: 9.9044

>T216480 WP_002286801.1 NZ_CP083473:489423-489764 [Enterococcus faecium]
VSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRKTMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQ
LKSLDFKERKLKKIENLPLQDMAKIDQIIQYMF

Download         Length: 342 bp

>T216480 NZ_CP083473:489423-489764 [Enterococcus faecium]
GTGTCAGAAGAAAGAATTTATATTCCTAAAAAAGGTGATATTGTATGGATTGATTTTGATCCCTCCGTAGGAAAAGAAAT
TCAAAAAAGACGACCAGGACTAGTCGTCTCTCGTTATGAATTTAACCGTAAAACAATGTTTTCGGTCATATGTCCAATTA
CTTCCACTATAAAGAATTTACCGACACGTTACTCATTACCTGAAGAGCTAGATATAAAAGGACAAGTAATTATTACACAA
TTAAAATCTCTTGATTTTAAGGAACGAAAACTCAAAAAAATAGAAAACTTACCATTGCAAGACATGGCTAAAATTGATCA
GATTATACAGTATATGTTTTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8523.39 Da        Isoelectric Point: 3.9078

>AT216480 WP_002323011.1 NZ_CP083473:489193-489423 [Enterococcus faecium]
MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLVPKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL

Download         Length: 231 bp

>AT216480 NZ_CP083473:489193-489423 [Enterococcus faecium]
ATGCTTGTTACAAAATCTAGATTACAAGGAAGTTCTGTAGTGGTAACTTTACCTTCTGATAACGGAAAGAAACCTTCAGA
AAACCAAGAATATATCGTTGTGTATTCTGATGATGGAACAATTACGTTAGTTCCAAAAATTGAAGATCCATTTAGTGGTG
GGGAAGAGGCCGAGTATTATGAGAAAGACGAATGGGAAGATTTAACTCCTGAAGGGAGAGAAATTTTATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FD66

Antitoxin

Source ID Structure
AlphaFold DB A0A828ZZN9

References