Detailed information of TA system TA216477

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 355069..355651
• Replicon: plasmid p_TS293_3
• Accession: NZ_CP083457
• Organism: Deinococcus taeanensis strain TS293

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: LAJ19_RS17430
• Protein ID: WP_225523759.1
• Coordinates: 355301..355651 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: LAJ19_RS17425
• Protein ID: WP_225523758.1
• Coordinates: 355069..355314 (+)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LAJ19_RS17385 (LAJ19_17385)	350801..351688	-	888	WP_225523750.1	lysophospholipid acyltransferase family protein	-
LAJ19_RS17390 (LAJ19_17390)	351820..352071	-	252	WP_225523751.1	hypothetical protein	-
LAJ19_RS17395 (LAJ19_17395)	352068..352313	-	246	WP_225523752.1	FAD-dependent oxidoreductase	-
LAJ19_RS17400 (LAJ19_17400)	352628..353200	+	573	WP_225523753.1	carboxymuconolactone decarboxylase family protein	-
LAJ19_RS17405 (LAJ19_17405)	353406..353672	-	267	WP_225523754.1	hypothetical protein	-
LAJ19_RS17410 (LAJ19_17410)	353669..354358	-	690	WP_225523755.1	hypothetical protein	-
LAJ19_RS17415 (LAJ19_17415)	354355..354729	-	375	WP_225523756.1	hypothetical protein	-
LAJ19_RS17425 (LAJ19_17425)	355069..355314	+	246	WP_225523758.1	ribbon-helix-helix domain-containing protein	Antitoxin
LAJ19_RS17430 (LAJ19_17430)	355301..355651	+	351	WP_225523759.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
LAJ19_RS17435 (LAJ19_17435)	355665..356600	+	936	WP_225523760.1	tyrosine-type recombinase/integrase	-
LAJ19_RS17440 (LAJ19_17440)	356601..357476	-	876	WP_225523761.1	Fic family protein	-
LAJ19_RS17445 (LAJ19_17445)	357487..357690	-	204	WP_155300869.1	hypothetical protein	-
LAJ19_RS17450 (LAJ19_17450)	357726..357875	-	150	WP_225523762.1	hypothetical protein	-
LAJ19_RS17455 (LAJ19_17455)	357933..358172	-	240	WP_225523763.1	hypothetical protein	-
LAJ19_RS17460 (LAJ19_17460)	358183..358905	-	723	WP_225523764.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..430426	430426

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-116)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12413.25 Da
• Isoelectric Point: 9.8890

>T216477 WP_225523759.1 NZ_CP083457:355301-355651 [Deinococcus taeanensis]
MQRGDIYIADLDPARASEANKRRPVVIVSANSLNRTVHRLGAGAITVVPLTSNVTRVYDFQVLLPAEQTGLDQDSKAQAE
QIRTISFSRLGPAPVGAVPAALMGQLDAALRLHLSL

Nucleotide

• Length: 351 bp

>T216477 NZ_CP113074:1780644-1780826 [Acinetobacter baumannii]
GTGAAAAGTCTGGATTTAATCAAAATGATTGAAGCAGATGGTTGGTATGAGGTTAGGGTTTCAGGAAGTCATCATCACTT
TAAACACCCAACCAAAAAGGGGTTAGTTACAATCCCACATCCTAAAAAGGATTTACCAAACGGAACTGTTAAAAGCATTT
TGAAACAAGCGGGTCTAAATTGA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 8953.81 Da
• Isoelectric Point: 4.0800

>AT216477 WP_225523758.1 NZ_CP083457:355069-355314 [Deinococcus taeanensis]
MTVGKMSVSLDASLLEFMAHYQETHQLRSRSEVVAQALTLLRDQELEAQYAAALSEWQDSSDADLWEHTAADGLDGTDAA
R

Nucleotide

• Length: 246 bp

>AT216477 NZ_CP113074:1780919-1781323 [Acinetobacter baumannii]
ATGTTATATCCAATTGCAATTGAACGAGGATCAGATACTGAGGCATTTGGTGTCACTGTTCCTGATATTCCAGGTTGTTT
TAGTGCTGGTGACACACTTGAAGAAGCTATTGAGAATGTTAAAGAAGCTATTTCAGGCCATTTAGAAATATTGGCTGAAG
ATGGTGAGGAAATCCCATTAGCTTCCGAACTAGTTAAATTTGTCGATGATCCTGAATATAAAGGAATGATCTGGGCGGTT
ACCGAAGTTGATGTTAGTCGTTATCTGGGTAAACCAGAAAAAATCAATGTTACTTTACCAAGCCGTTTGATTCGTAAAAT
TGATGAGAATGTAGGTAAAGGTAAGAGATATACTACTCGATCGGCTTTCTTGGCTGCTGGTGCTGAAAAACTTTTACATG
CATAG