Detailed information of TA system TA216469

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 44114..44640
• Replicon: plasmid unnamed1
• Accession: NZ_CP083449
• Organism: Pantoea eucrina strain XL123

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A2S5K783
• Locus tag: LAC65_RS16350
• Protein ID: WP_040113359.1
• Coordinates: 44353..44640 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: LAC65_RS16345
• Protein ID: WP_040113358.1
• Coordinates: 44114..44353 (+)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LAC65_RS16325 (LAC65_16325)	39591..41687	-	2097	WP_192415538.1	EAL domain-containing protein	-
LAC65_RS16330 (LAC65_16330)	42010..42393	-	384	WP_040113356.1	lysozyme inhibitor LprI family protein	-
LAC65_RS16335 (LAC65_16335)	42443..43492	-	1050	WP_040113357.1	tyrosine-type recombinase/integrase	-
LAC65_RS16340 (LAC65_16340)	43993..44089	-	97	Protein_43	hypothetical protein	-
LAC65_RS16345 (LAC65_16345)	44114..44353	+	240	WP_040113358.1	type II toxin-antitoxin system antitoxin RelB	Antitoxin
LAC65_RS16350 (LAC65_16350)	44353..44640	+	288	WP_040113359.1	type II toxin-antitoxin system mRNA interferase RelE	Toxin
LAC65_RS16365 (LAC65_16365)	46793..47155	+	363	WP_040113360.1	hypothetical protein	-
LAC65_RS16370 (LAC65_16370)	47166..49265	+	2100	WP_040113361.1	pyrroloquinoline quinone-dependent dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..107755	107755

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-83)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-79)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11163.15 Da
• Isoelectric Point: 10.1967

>T216469 WP_040113359.1 NZ_CP083449:44353-44640 [Pantoea eucrina]
MAYLLDFDERALKEWRKLGSTVREQLKKKLAEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Nucleotide

• Length: 288 bp

>T216469 NZ_CP113052:c1823544-1823356 [Staphylococcus aureus]
ATGTCTTTACATTTTGCAATTCTGTTTTGGCTAGCATTAATTTTCTTAGTTGCCGCTACGTTTATACTCGTATTAATGAA
AAAAACTAGCAAAGAATCTAAAAAAGAGTCCTATTTAAGTTTCACTGTCATTCTCTATATTTTTGGATTCGCTATATTAA
TATACACATTTATATTTGGTGTGCTATAA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 9085.50 Da
• Isoelectric Point: 4.5348

>AT216469 WP_040113358.1 NZ_CP083449:44114-44353 [Pantoea eucrina]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIAENERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Nucleotide

• Length: 240 bp

>AT216469 NZ_CP113052:c1823833-1823573 [Staphylococcus aureus]
ATGAATAACATTTTGTTAAATGCTATCAATATAGTTATTACTACCACTTTTGTTATCTTTAATATTTTAATCACATATAA
TAAAGATTTAGATGATTTATGTTGGCTCCTGCCTGGTATTATCATTTGTGGTGTGATACTCATCGTATCCTTTACCATTG
CAATGATAACTAAAAACTGGTTAAGTGAAATATTATTTTTTATAAATATCGTACTCGTTCTCTATTACATTTATCCTATT
TTTTATAGTTTTATAGGTTAA

Structures

Toxin

• AlphaFold DB: A0A2S5K783