Detailed information of TA system TA216468

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 3382864..3384434
• Replicon: chromosome
• Accession: NZ_CP083448
• Organism: Pantoea eucrina strain XL123

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: LAC65_RS16085
• Protein ID: WP_039381476.1
• Coordinates: 3382864..3384183 (-)
• Length: 440 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: LAC65_RS16090
• Protein ID: WP_039381479.1
• Coordinates: 3384183..3384434 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LAC65_RS16065 (LAC65_16065)	3378950..3379627	-	678	WP_039381461.1	6-phosphogluconate phosphatase	-
LAC65_RS16070 (LAC65_16070)	3379777..3381090	+	1314	WP_039381465.1	NCS2 family permease	-
LAC65_RS16075 (LAC65_16075)	3381121..3381882	-	762	WP_039381469.1	4'-phosphopantetheinyl transferase	-
LAC65_RS16080 (LAC65_16080)	3382064..3382654	-	591	WP_039381472.1	malonic semialdehyde reductase	-
LAC65_RS16085 (LAC65_16085)	3382864..3384183	-	1320	WP_039381476.1	type II toxin-antitoxin system HipA family toxin	Toxin
LAC65_RS16090 (LAC65_16090)	3384183..3384434	-	252	WP_039381479.1	helix-turn-helix domain-containing protein	Antitoxin
LAC65_RS16095 (LAC65_16095)	3384787..3386151	-	1365	WP_039381482.1	tRNA uridine-5-carboxymethylaminomethyl(34) synthesis GTPase MnmE	-
LAC65_RS16100 (LAC65_16100)	3386256..3387902	-	1647	WP_039381485.1	membrane protein insertase YidC	-
LAC65_RS16105 (LAC65_16105)	3387905..3388162	-	258	WP_071668252.1	membrane protein insertion efficiency factor YidD	-
LAC65_RS16110 (LAC65_16110)	3388126..3388485	-	360	WP_071668251.1	ribonuclease P protein component	-
LAC65_RS16115 (LAC65_16115)	3388501..3388641	-	141	WP_003849659.1	50S ribosomal protein L34	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (4-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (13-65)

Sequences

Toxin

Protein

• Length: 440 a.a.
• Molecular weight: 48903.78 Da
• Isoelectric Point: 6.6109

>T216468 WP_039381476.1 NZ_CP083448:c3384183-3382864 [Pantoea eucrina]
MAELDVYMNGYKVGVFSRAGTGAHAFQYAEAWLNQTGSRPVSLSMPLRRQPYQGNEVYNFFDNLLPDNPEIRGRILARYN
ADSTQPFDLLARIGADTVGALQLLPKDAAPGDIRTIDYKALSDRDLAKILTGYKAGAPLGMIEEIDDFRISLAGAQEKTA
LLFSNDCWCQPRGTSPTTHILKLPIGKIESHSYSLDLSDSVENEFLCTQLAKEFGLPVPDCFILHINDIKALAVKRFDRR
YASDGSWIMRLPQEDFCQVLNVPSARKYENQGGPGIGDIMQTLLGSANAEQDRYHFMKAQVLFWLLAATDGHAKNFSVFI
EPEGRFRLTPFYDILSVYPVFGGRGLNRRDARLAMGLTSSKGKKYAIEQIFPRHFFQTAKAVGFERSVMEEILTELANSV
DVVIERVTRQLPAGFPDANSSAILEGLKARSARLIAGWE

Nucleotide

• Length: 1320 bp

>T216468 NZ_CP113052:c650204-649938 [Staphylococcus aureus]
ATGAGCAATTACACGGTTAAGATTAAAAATTCAGCGAAATCAGATTTAAAGAAAATAAAACATTCTTATTTAAAGAAGTC
ATTTTTAGAAATTGTTGAGACTTTAAAAAATGATCCGTATAAAATAACACAATCTTTTGAAAAATTAGAGCCTAAATATT
TAGAGCGATATTCAAGAAGAATTAACCATCAGCACAGGGTCGTCTATACCGTAGATGATCGAAATAAAGAAGTATTAATA
CTATCGGCATGGTCACATTATGATTAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9360.70 Da
• Isoelectric Point: 9.9498

>AT216468 WP_039381479.1 NZ_CP083448:c3384434-3384183 [Pantoea eucrina]
MKIINVSQLSAHLRDVRLSKKLSQGKVASKVGIRQDTVSSFELQPGTSRLETFFRILSALELELEVKPKGSDTQSSEGWK
EEW

Nucleotide

• Length: 252 bp

>AT216468 NZ_CP113052:c650461-650204 [Staphylococcus aureus]
ATGATTATCACTAGCCCTACAGAAGCGAGAAAAGATTTTTATCAATTACTAAAAAATGTTAATAATAATCACGAACCAAT
TTATATTAGTGGCAATAATGCCGAAAATAATGCTGTGATTATAGGTTTAGAAGATTGGAAAAGTATACAAGAGACAATAT
ATCTTGAATCTACTGGTACAATGGACAAAGTAAGAGAAAGAGAAAAAGATAATAGTGGTACAACAAATATAGATGATATT
GATTGGGATAATCTTTAA