21646

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) itaRT/DUF1778(antitoxin)
Location 417089..417933 Replicon chromosome
Accession NC_008258
Organism Shigella flexneri 5 str. 8401

Toxin (Protein)


Gene name itaT Uniprot ID A0A4P7TPN5
Locus tag SFV_RS02205 Protein ID WP_005060796.1
Coordinates 417089..417487 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name itaR Uniprot ID A0A4P7TQW7
Locus tag SFV_RS02215 Protein ID WP_024260230.1
Coordinates 417622..417933 (-) Length 104 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
SFV_RS02190 412823..414193 - 1371 WP_001000961.1 MFS transporter -
SFV_RS02195 414587..415861 + 1275 WP_005105027.1 sel1 repeat family protein -
SFV_RS27840 415854..416087 + 234 WP_128600802.1 hypothetical protein -
SFV_RS02200 416098..417033 + 936 WP_005105029.1 sel1 repeat family protein -
SFV_RS02205 417089..417487 - 399 WP_005060796.1 GNAT family N-acetyltransferase Toxin
SFV_RS02215 417622..417933 - 312 WP_024260230.1 DUF1778 domain-containing protein Antitoxin
SFV_RS02220 418118..419008 - 891 Protein_418 protoheme IX farnesyltransferase -
SFV_RS02225 419020..419349 - 330 WP_000019869.1 cytochrome o ubiquinol oxidase subunit IV -
SFV_RS02230 419349..419963 - 615 WP_000179819.1 cytochrome o ubiquinol oxidase subunit III -
SFV_RS02235 419953..421944 - 1992 Protein_421 cytochrome o ubiquinol oxidase subunit I -
SFV_RS02240 421966..422463 - 498 Protein_422 COX aromatic rich motif-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 410488..418489 8001


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(52-112)

Antitoxin

Vibrio phage ICP1, Orf50

(15-97)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14335.69 Da        Isoelectric Point: 9.1674

>T21646 WP_005060796.1 NC_008258:c417487-417089 [Shigella flexneri 5 str. 8401]
VRNSDCAAKALIDRQSGELIGICTFTAYSLERQRVSGVLQGSQPSEIGVVRLVMLGGARKYQKRGFGQDLLCDFFEHVKK
IHPALPIKGVYLDADPAAINFYARLGFVQLSATPNAFGAVPMFLAIQHILAA

Download         Length: 399 bp

>T21646 NC_008258:c417487-417089 [Shigella flexneri 5 str. 8401]
GTGCGTAATAGCGACTGTGCGGCTAAAGCACTTATTGACAGACAAAGTGGTGAACTGATTGGCATCTGTACTTTTACGGC
ATATTCGCTGGAAAGACAACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAAATTGGTGTTGTCAGATTAGTCA
TGTTGGGGGGAGCGCGGAAGTATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAAAA
ATTCACCCGGCATTACCAATTAAAGGGGTTTATCTTGATGCTGACCCTGCCGCCATTAACTTTTATGCTCGTCTCGGCTT
TGTTCAGCTTTCAGCGACACCAAATGCTTTTGGTGCTGTACCTATGTTTTTGGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin


Download         Length: 104 a.a.        Molecular weight: 11440.14 Da        Isoelectric Point: 10.3388

>AT21646 WP_024260230.1 NC_008258:c417933-417622 [Shigella flexneri 5 str. 8401]
MLGKTAALYDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIT
EPAQPTLALKALMKRKNSDGRQA

Download         Length: 312 bp

>AT21646 NC_008258:c417933-417622 [Shigella flexneri 5 str. 8401]
ATGCTTGGAAAAACAGCCGCTCTATACGATGTCGATAAAACGCTGAAAAATGCGCGGGTCGAACTCAAAACTAGTCCCGA
CGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCTGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCACT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TPN5

Antitoxin

Source ID Structure
AlphaFold DB A0A4P7TQW7

References