Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-RelB
Location 2954546..2955132 Replicon chromosome
Accession NZ_CP083445
Organism Klebsiella pneumoniae strain KP294

Toxin (Protein)


Gene name doc Uniprot ID W8VD46
Locus tag LAV94_RS14355 Protein ID WP_002920800.1
Coordinates 2954764..2955132 (+) Length 123 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID W9B1V1
Locus tag LAV94_RS14350 Protein ID WP_004174006.1
Coordinates 2954546..2954767 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LAV94_RS14330 (2950703) 2950703..2951629 + 927 WP_002920807.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
LAV94_RS14335 (2951626) 2951626..2952903 + 1278 WP_004188292.1 branched chain amino acid ABC transporter permease LivM -
LAV94_RS14340 (2952900) 2952900..2953667 + 768 WP_002920803.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -
LAV94_RS14345 (2953669) 2953669..2954382 + 714 WP_004145133.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF -
LAV94_RS14350 (2954546) 2954546..2954767 + 222 WP_004174006.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
LAV94_RS14355 (2954764) 2954764..2955132 + 369 WP_002920800.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
LAV94_RS14360 (2955405) 2955405..2956721 + 1317 WP_004174008.1 sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB -
LAV94_RS14365 (2956828) 2956828..2957715 + 888 WP_014906831.1 sn-glycerol-3-phosphate ABC transporter permease UgpA -
LAV94_RS14370 (2957712) 2957712..2958557 + 846 WP_004145129.1 sn-glycerol-3-phosphate ABC transporter permease UgpE -
LAV94_RS14375 (2958559) 2958559..2959629 + 1071 WP_004188298.1 sn-glycerol-3-phosphate import ATP-binding protein UgpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 2949532..2960366 10834


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Fido domain

(13-86)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-50)


Sequences

Toxin        


Download         Length: 123 a.a.        Molecular weight: 13552.92 Da        Isoelectric Point: 8.6410

>T216453 WP_002920800.1 NZ_CP083445:2954764-2955132 [Klebsiella pneumoniae]
MTLQIISAEEIIQFHDRLLRVTPGVAGMPDPGRAEAIMYRVLNKIEYEGVTDVWRLAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGIILPANPDFVGMTVEAAAGQLTLEQIVARLRG

Download         Length: 369 bp

>T216453 NZ_CP113051:c1660417-1660241 [Staphylococcus aureus]
TTGGATAGATGGTGGCTATCTGAGTATAAGGAGGTGGTGCCTATGGTGGCATTACTGAAATCTTTAGAAAGGAGACGCCT
AATGATTACAATTAGTACCATGTTGCAGTTTGGTTTATTCCTTATTGCATTGATAGGTCTAGTAATCAAGCTTATTGAAT
TAAGCAATAAAAAATAA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8151.15 Da        Isoelectric Point: 4.7769

>AT216453 WP_004174006.1 NZ_CP083445:2954546-2954767 [Klebsiella pneumoniae]
MRTVNYSEARQNLADVLESAVTGVPVTITRRGHKSAVIISAEEFERYQAARMDDEFAAIMAVHGDEIRELADK

Download         Length: 222 bp

>AT216453 NZ_CP113051:1660110-1660251 [Staphylococcus aureus]
ATATATAGAAAAAGGGCAACATGCGCAAACATGTTACCCTAGTGAGCCCGTTAAAAAGACGGTGGCTATTTTAGATTAAA
GATTAAATTAATAACCATTTAACCATCGAAACCAGCCAAAGTTAGCGATGGTTATTTTTTAT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GUD1

Antitoxin

Source ID Structure
AlphaFold DB A0A5E5YJY7

References