Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 2552186..2552811 Replicon chromosome
Accession NZ_CP083445
Organism Klebsiella pneumoniae strain KP294

Toxin (Protein)


Gene name vapC Uniprot ID A0A483GDF6
Locus tag LAV94_RS12490 Protein ID WP_004187928.1
Coordinates 2552186..2552569 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag LAV94_RS12495 Protein ID WP_004150355.1
Coordinates 2552569..2552811 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LAV94_RS12475 (2549552) 2549552..2550454 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
LAV94_RS12480 (2550451) 2550451..2551086 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
LAV94_RS12485 (2551083) 2551083..2552012 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
LAV94_RS12490 (2552186) 2552186..2552569 - 384 WP_004187928.1 type II toxin-antitoxin system VapC family toxin Toxin
LAV94_RS12495 (2552569) 2552569..2552811 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
LAV94_RS12500 (2553005) 2553005..2553922 + 918 WP_004187929.1 alpha/beta hydrolase -
LAV94_RS12505 (2553936) 2553936..2554877 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
LAV94_RS12510 (2554922) 2554922..2555359 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
LAV94_RS12515 (2555356) 2555356..2556216 - 861 WP_004146232.1 virulence factor BrkB family protein -
LAV94_RS12520 (2556210) 2556210..2556809 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-110)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14407.64 Da        Isoelectric Point: 7.3178

>T216452 WP_004187928.1 NZ_CP083445:c2552569-2552186 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFTGIPGVVTPYEIHPE

Download         Length: 384 bp

>T216452 NZ_CP113051:c1543437-1543285 [Staphylococcus aureus]
ATGTCTAAAGACAAAGATCCAAAATTAAATTATCATGAAGAAGAAAACAGTATGGTAACGGATTTTGAAGATTTAAAAGA
ATTAGGTAAAGAAATGGAACAAATTTCTGATCAAAATGATCAAGAAAAGAATTCTGAAGAAGACAGTCAGTAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT216452 WP_004150355.1 NZ_CP083445:c2552811-2552569 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT216452 NZ_CP113051:c1544061-1543462 [Staphylococcus aureus]
ATGGCAATGAACTTTAAAGTCTTTGACAATAGTCAACTTGTAGCAGAATATGCTGCTGATATTATTAGAAAGCAATTTAA
CAATAATCCTACTACAATTGCAGGTTTTCATTTAGATACAGATCAAGCGCCAGTTCTAGATGAATTAAAGAAAAATGTTG
AAAAACATGCTGTTGATTTTAGCCAAATAAATATTTTAGATTATGACGATAAAAAATCATATTTCGAAGCGTTAGGTGTA
CCAGCAGGTCAAGTTTATCCAATTGCTTATGAAAAAGATGCAATCGAATTAATCGCTGATAAGATTAAAACTAAAGAAAA
TAAAGGGAAATTAACATTACAAGTTGTTTCTATCGATGAGCAAGGTAAGTTAAATATTAGTATTCGTCAAGGACTAATGG
AAGCAAGAGAAATTTTCTTAGTAGTGACAGGTGCTAATAAACGAGATGTAGTTGAAAAATTATATCAAGAAAATGGTAAA
ACAAGCTTCGAACCAGCCGATTTAAAAGCACATAGAATGGTAAATGTTATTCTTGATAAAGAAGCGGCTGCAGGTTTACC
TGAAGATGTTAAAGCTTACTTTACGTCACGCTTTGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A483GDF6

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References