Detailed information of TA system TA216451

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mosAT/AbiEii-AbiEi
• Location: 2229771..2231452
• Replicon: chromosome
• Accession: NZ_CP083445
• Organism: Klebsiella pneumoniae strain KP294

Toxin (Protein)

• Gene name: mosT
• Uniprot ID: W8V1Q6
• Locus tag: LAV94_RS11000
• Protein ID: WP_002885155.1
• Coordinates: 2229771..2230688 (-)
• Length: 306 a.a.

Antitoxin (Protein)

• Gene name: mosA
• Uniprot ID: A0A2L1C4M5
• Locus tag: LAV94_RS11005
• Protein ID: WP_004192584.1
• Coordinates: 2230694..2231452 (-)
• Length: 253 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LAV94_RS10980 (2225077)	2225077..2226597	+	1521	WP_002885173.1	sugar ABC transporter ATP-binding protein	-
LAV94_RS10985 (2226594)	2226594..2227592	+	999	WP_002885172.1	ribose ABC transporter permease	-
LAV94_RS10990 (2227623)	2227623..2228564	+	942	WP_002885156.1	ABC transporter substrate-binding protein	-
LAV94_RS10995 (2228639)	2228639..2229646	+	1008	WP_004177805.1	carbohydrate kinase family protein	-
LAV94_RS11000 (2229771)	2229771..2230688	-	918	WP_002885155.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
LAV94_RS11005 (2230694)	2230694..2231452	-	759	WP_004192584.1	type IV toxin-antitoxin system AbiEi family antitoxin	Antitoxin
LAV94_RS11010 (2231538)	2231538..2232266	-	729	WP_002885152.1	response regulator transcription factor	-
LAV94_RS11015 (2232539)	2232539..2234686	+	2148	WP_012737173.1	formate dehydrogenase H subunit alpha, selenocysteine-containing	-
LAV94_RS11020 (2234916)	2234916..2235602	+	687	WP_004151733.1	tetratricopeptide repeat protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (14-278)

Antitoxin

• Transcriptional regulator AbiEi antitoxin, N-terminal domain (1-93)

Sequences

Toxin

Protein

• Length: 306 a.a.
• Molecular weight: 34687.15 Da
• Isoelectric Point: 5.4707

>T216451 WP_002885155.1 NZ_CP083445:c2230688-2229771 [Klebsiella pneumoniae]
MDIQAPYYRQVALLMQVLPYVAVEREFALKGGTAINLFIRDFPRLSVDIDLAWVPLESRAIALPHIRDALARIAANLQQQ
AGMSAVLQANRSDEMRVIVTTDSAQIKIEVSPVARGTLYPPQEREVVGAVEDVFGYASLPVVSLPDLYGGKLCAALDRQH
PRDFYDVKLLLDAQELDRPIFNGFIVYLLSHNRPLAEVLNPRWKDIAEPFYREFSGMTFETIALEELTAVPNRMIAALKS
CFTQQDVDFLLSFKRGEPDWRLAPEMRIQDLPAVQWKLRNIHQMPAIKRAESLDKLEKVLAEWRS

Nucleotide

• Length: 918 bp

>T216451 NZ_CP113051:428256-428576 [Staphylococcus aureus]
ATGAATTGGGAAATTAAAGATTTGATGTGTGATATTGAAGTGATAAAAGAAAAAATCAATGATGTAGCTATCAAACATGG
TTGGTTTGTTGAAGATAAATTTGTCAAAAATGAATTAGAAACAAAACAGGAACATATTAATTTTTCTGCTAGCTATTTAG
AACATCGTATACAAAATGAACATACAGTTGAGTTATTACAGGTGTACTTAAAAGAATTCGGTGAACTTATACAAAAGTTT
CATGAAATAGAAAAAGCATCATCTGATGGAACTTTGGCGACGGAATCAGATGACGCAAAGAAATTAAAAATTACAGAGTA
A

Antitoxin

Protein

• Length: 253 a.a.
• Molecular weight: 28582.94 Da
• Isoelectric Point: 10.0870

>AT216451 WP_004192584.1 NZ_CP083445:c2231452-2230694 [Klebsiella pneumoniae]
MATKLNWLLQNSAPGDVILQSWLSQHAISPSLAFKYAQSGWLKKRGNGVYARAGREPEWNDAFACLQNQLAAPVYVAGLS
SLVWQGRSHYLQLKQNQCWLSVENKALLPKWFREFPGVEWIVISGKKLPVLDDRYRVTLDIKGKKLIGSAPELAAYELLS
AVPGTLSFNHAAELFQGLVNLNPRKVEYLLSVSQSVQAKRLYLFFASFYEHGWLKRIDSQKIDLGAGKRQIVENGKFNAQ
YQITVPERFQKE

Nucleotide

• Length: 759 bp

>AT216451 NZ_CP113051:428050-428253 [Staphylococcus aureus]
ATGAACAAAAAAGAAGTAATAGAGCACGTGGTTTATCAACTTGAGTTAAGTATCATGAATAATTTTGAAAGTAATGAACA
CACGGAATATGTTGATGGTATTGAAGTGGTTTCAGAGATCAGTCGTGAAAAGCACTTAGAATTGATAATGAAATGGTGCG
CACAAGAATTAAAGAATAATTTTCAATTAGAAAAAGGAGAATAA

Structures

Toxin

• AlphaFold DB: A0A0H3GH11

Antitoxin

• AlphaFold DB: A0A2L1C4M5