Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 1958207..1959017 Replicon chromosome
Accession NZ_CP083445
Organism Klebsiella pneumoniae strain KP294

Toxin (Protein)


Gene name KacT Uniprot ID A0A483G813
Locus tag LAV94_RS09645 Protein ID WP_023279404.1
Coordinates 1958207..1958740 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag LAV94_RS09650 Protein ID WP_002887278.1
Coordinates 1958751..1959017 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LAV94_RS09640 (1957038) 1957038..1958159 + 1122 WP_002887282.1 cupin domain-containing protein -
LAV94_RS09645 (1958207) 1958207..1958740 - 534 WP_023279404.1 type II toxin-antitoxin system toxin KacT Toxin
LAV94_RS09650 (1958751) 1958751..1959017 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
LAV94_RS09655 (1959120) 1959120..1960553 - 1434 WP_004192214.1 Cu(+)/Ag(+) sensor histidine kinase -
LAV94_RS09660 (1960543) 1960543..1961226 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
LAV94_RS09665 (1961398) 1961398..1962783 + 1386 WP_004192218.1 efflux transporter outer membrane subunit -
LAV94_RS09670 (1962801) 1962801..1963145 + 345 WP_004192220.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19809.71 Da        Isoelectric Point: 6.2369

>T216449 WP_023279404.1 NZ_CP083445:c1958740-1958207 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDKNSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T216449 NZ_CP113049:2906210-2906461 [Staphylococcus aureus]
ATGAATAACCGCGAACAAATTGAACAGTCCGTTATAAGTGCTAGTGCGTATAACGGCAATGACACAGAGGGATTACTAAA
AGAGATTGAGGACGTGTATAAGAAAGCGCAAGCGTTTGATGAAATACTTAAGGGTTTACCTAATGCTATGCAAGATGCAC
TCAAAGAAGATATTGGTCTTGATGAAGCAGTAGGGATTATGACGGGGCAAGTGGTCTATAAATATGAGGAGGAGCAGGAA
AATGAGTATTAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT216449 WP_002887278.1 NZ_CP083445:c1959017-1958751 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT216449 NZ_CP113049:2906451-2906624 [Staphylococcus aureus]
ATGAGTATTAGTGTAGGAGATAAAGTATATAACCATGAAACAAACGAAAGTCTAGAGATTGTGCAATTGGTCGGAGATAT
TAGAGATACACATTATAAACTGTCTGATGATTCAGTTATTAGCATTATAGATTTTATTACTAAACCAATTTATCTAATTA
AGGGGGACGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A483G813

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References