Detailed information of TA system TA216426

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 786669..787339
• Replicon: chromosome
• Accession: NZ_CP083443
• Organism: Klebsiella pneumoniae strain KP1711R

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: Q6U619
• Locus tag: K9870_RS03915
• Protein ID: WP_004213072.1
• Coordinates: 786896..787339 (+)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q6U620
• Locus tag: K9870_RS03910
• Protein ID: WP_004213073.1
• Coordinates: 786669..786899 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K9870_RS03885 (782892)	782892..783791	-	900	WP_004225022.1	nucleotide-binding protein	-
K9870_RS03890 (783781)	783781..784071	-	291	WP_004213078.1	hypothetical protein	-
K9870_RS03895 (784423)	784423..784629	+	207	WP_004213077.1	hypothetical protein	-
K9870_RS03900 (784619)	784619..784912	-	294	WP_119062594.1	korC	-
K9870_RS03905 (784928)	784928..786061	-	1134	WP_004213075.1	DUF3800 domain-containing protein	-
K9870_RS03910 (786669)	786669..786899	+	231	WP_004213073.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
K9870_RS03915 (786896)	786896..787339	+	444	WP_004213072.1	type II toxin-antitoxin system VapC family toxin	Toxin
K9870_RS03920 (787488)	787488..787739	+	252	WP_186987481.1	hypothetical protein	-
K9870_RS03925 (787762)	787762..788066	-	305	Protein_771	transposase	-
K9870_RS03930 (788483)	788483..789117	+	635	Protein_772	mucoid phenotype regulator RmpA2	-
K9870_RS03935 (789635)	789635..790038	-	404	Protein_773	GAF domain-containing protein	-
K9870_RS03940 (790129)	790129..791049	-	921	WP_004213934.1	DUF1471 domain-containing protein	-
K9870_RS03945 (791098)	791098..791589	-	492	WP_011251327.1	DM13 domain-containing protein	-
K9870_RS03950 (791652)	791652..791918	-	267	WP_147021650.1	carboxymuconolactone decarboxylase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	aac(3)-IId / blaSHV-187	fimA / fimI / fimC / fimD / fimD / fimF / fimG / rmpA / iutA / iucD / iucC / iucB / iucA	580689..808269	227580

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 16006.74 Da
• Isoelectric Point: 9.3642

>T216426 WP_004213072.1 NZ_CP083443:786896-787339 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVKKAALCRVIS

Nucleotide

• Length: 444 bp

>T216426 NZ_CP113044:c1762991-1762803 [Staphylococcus aureus]
ATGTCTTTACATTTTGCAATTCTGTTTTGGCTAGCATTAATTTTCTTAGTTGCCGCTACGTTTATACTCGTATTAATGAA
AAAAACTAGCAAAGAATCTAAAAAAGAGTCCTATTTAAGTTTCACTGTCATTCTCTATATTTTTGGATTCGCTATATTAA
TATACACATTTATATTTGGTGTGCTATAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8677.73 Da
• Isoelectric Point: 4.4563

>AT216426 WP_004213073.1 NZ_CP083443:786669-786899 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRAAPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT216426 NZ_CP113044:c1763280-1763020 [Staphylococcus aureus]
ATGAATAACATTTTGTTAAATGCTATCAATATAGTTATTACTACCACTTTTGTTATCTTTAATATTTTAATCACATATAA
TAAAGATTTAGATGATTTATGTTGGCTCCTGCCTGGTATTATCATTTGTGGTGTGATACTCATCGTATCCTTTACCATTG
CAATGATAACTAAAAACTGGTTAAGTGAAATATTATTTTTTATAAATATCGTACTCGTTCTCTATTACATTTATCCTATT
TTTTATAGTTTTATAGGTTAA

Structures

Toxin

• AlphaFold DB: Q6U619

Antitoxin

• AlphaFold DB: Q6U620