Detailed information of TA system TA216421

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-StbC
• Location: 3207264..3207934
• Replicon: chromosome
• Accession: NZ_CP083442
• Organism: Pseudomonas chlororaphis strain L5734

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: K9V45_RS14665
• Protein ID: WP_025808439.1
• Coordinates: 3207506..3207934 (+)
• Length: 143 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A3G7DZE9
• Locus tag: K9V45_RS14660
• Protein ID: WP_009046453.1
• Coordinates: 3207264..3207509 (+)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K9V45_RS14640 (K9V45_14640)	3203321..3204298	+	978	WP_025808435.1	taurine ABC transporter substrate-binding protein	-
K9V45_RS14645 (K9V45_14645)	3204409..3205203	+	795	WP_226305526.1	taurine ABC transporter ATP-binding subunit	-
K9V45_RS14650 (K9V45_14650)	3205200..3206039	+	840	WP_226305527.1	taurine ABC transporter permease TauC	-
K9V45_RS14655 (K9V45_14655)	3206285..3207124	+	840	WP_226305528.1	taurine dioxygenase	-
K9V45_RS14660 (K9V45_14660)	3207264..3207509	+	246	WP_009046453.1	plasmid stabilization protein	Antitoxin
K9V45_RS14665 (K9V45_14665)	3207506..3207934	+	429	WP_025808439.1	type II toxin-antitoxin system VapC family toxin	Toxin
K9V45_RS14670 (K9V45_14670)	3208064..3209101	-	1038	WP_226305529.1	L-glyceraldehyde 3-phosphate reductase	-
K9V45_RS14675 (K9V45_14675)	3209696..3210097	-	402	WP_025808441.1	type II toxin-antitoxin system HicB family antitoxin	-
K9V45_RS14680 (K9V45_14680)	3210127..3210309	-	183	WP_009041685.1	type II toxin-antitoxin system HicA family toxin	-
K9V45_RS14685 (K9V45_14685)	3210434..3211324	-	891	WP_226305530.1	LysR family transcriptional regulator	-
K9V45_RS14690 (K9V45_14690)	3211455..3212195	+	741	WP_226305531.1	SDR family oxidoreductase	-
K9V45_RS14695 (K9V45_14695)	3212252..3212740	+	489	WP_226305532.1	DoxX family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-124)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 143 a.a.
• Molecular weight: 15911.44 Da
• Isoelectric Point: 5.4927

>T216421 WP_025808439.1 NZ_CP083442:3207506-3207934 [Pseudomonas chlororaphis]
MIVLDTNVLSELMRPQPDTAVLLWIEAQPVEDLYISAMTMAEILHGIARLPHGKRKQTLHASALAMFEEDFIDRIVPFGT
QAALYYAHLVSHRERSGQPINLVDTQIAAICRVHDAAIATRNTKDFTDTGLIVINPWLPLEI

Nucleotide

• Length: 429 bp

>T216421 NZ_CP113044:c210092-209883 [Staphylococcus aureus]
ATGATACATCAAAATACGATTTACACAGCGGGAATTGAAACAGAAGAACAAGTAAGTCAATTGACAGAACGCATTTCAAA
TATGATAGGTGTTCATCAAGTGAATATTAATATAATAGATGGTCAAGTAACTGTATCGTATGAGACACCAGCAAATTTGA
ATAGTATTGAAAAAGAAATCTATGATGAAGGATACAAAATTGTATTTTAG

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 9103.27 Da
• Isoelectric Point: 6.2314

>AT216421 WP_009046453.1 NZ_CP083442:3207264-3207509 [Pseudomonas chlororaphis]
MASLTIRNLDDHIKERLRIEAAHNGHSMEEEARLILRRALSRTADSQGLGSRIRARFAETGGVELELPERTEPARLVDLG
E

Nucleotide

• Length: 246 bp

>AT216421 NZ_CP113044:c210397-210104 [Staphylococcus aureus]
ATGACTGAACAAGATAATGCACATCATTCTGAACAAATAAAAACGAATCTTAAATCACGTTTAAATCGAATTGAAGGACA
AGTGAGAGCGATTAATCGCATGATTGAAGAAGATGTCTATTGTGATGATGTCCTTACGCAAATAAGAGCGACACGTTCGG
CGTTAAACAGTGTTGCGATAAAGTTATTAGAACAACATATGAAAAGTTGTATTATGAATAAAGTTAATCAAGGTGCTCAG
GAAGAGGCAATGGAAGAGTTATTAGTGACTTTTCAAAAATTGATTAAAGACTAA

Structures

Antitoxin

• AlphaFold DB: A0A3G7DZE9