Detailed information of TA system TA216420

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-RHH
• Location: 1977674..1978202
• Replicon: chromosome
• Accession: NZ_CP083442
• Organism: Pseudomonas chlororaphis strain L5734

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: K9V45_RS09060
• Protein ID: WP_226305123.1
• Coordinates: 1977903..1978202 (+)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: K9V45_RS09055
• Protein ID: WP_097302284.1
• Coordinates: 1977674..1977913 (+)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K9V45_RS09025 (K9V45_09025)	1972955..1973458	+	504	WP_025803965.1	inclusion body family protein	-
K9V45_RS09030 (K9V45_09030)	1973578..1974117	+	540	WP_226305119.1	AidA/PixA family protein	-
K9V45_RS09035 (K9V45_09035)	1974231..1974755	-	525	WP_226305120.1	DUF4142 domain-containing protein	-
K9V45_RS09040 (K9V45_09040)	1974894..1976243	-	1350	WP_226305121.1	sensor domain-containing diguanylate cyclase	-
K9V45_RS09045 (K9V45_09045)	1976355..1976993	-	639	WP_226305122.1	LysE family translocator	-
K9V45_RS09050 (K9V45_09050)	1977121..1977537	-	417	WP_106696692.1	fosfomycin resistance glutathione transferase	-
K9V45_RS09055 (K9V45_09055)	1977674..1977913	+	240	WP_097302284.1	CopG family ribbon-helix-helix protein	Antitoxin
K9V45_RS09060 (K9V45_09060)	1977903..1978202	+	300	WP_226305123.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
K9V45_RS09065 (K9V45_09065)	1978236..1978907	-	672	WP_226305124.1	epimerase	-
K9V45_RS09070 (K9V45_09070)	1979127..1979699	-	573	WP_226305125.1	TetR/AcrR family transcriptional regulator	-
K9V45_RS09075 (K9V45_09075)	1979897..1981045	-	1149	WP_226305126.1	L-threonine dehydrogenase	-
K9V45_RS09080 (K9V45_09080)	1981203..1982222	+	1020	WP_226305127.1	DUF4917 family protein	-
K9V45_RS09085 (K9V45_09085)	1982417..1982959	-	543	WP_007922237.1	XRE family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1969871..1979038	9167

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-89)

Antitoxin

• Ribbon-helix-helix protein, CopG (5-41)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11547.23 Da
• Isoelectric Point: 10.8916

>T216420 WP_226305123.1 NZ_CP083442:1977903-1978202 [Pseudomonas chlororaphis]
MQVEWLRTALKNLDDEAAYIAQDNPQAAAEFVKAILSSVQHLTRFPASGREGRLPGTREWVVPNRPYLIPYRIRQGRLQI
LRIFHTRRLPPAGWQDERQ

Nucleotide

• Length: 300 bp

>T216420 NZ_CP113044:c190489-190127 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAGTTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCACCAGAAAAATTAG

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 8550.58 Da
• Isoelectric Point: 4.2813

>AT216420 WP_097302284.1 NZ_CP083442:1977674-1977913 [Pseudomonas chlororaphis]
MSVMSLRLPDDVADTLAHLAKATGRSKSFLAVDALREYLAREAWQIDEIQKALTEAEAGDFASAEELDGVLNKWTGNAG

Nucleotide

• Length: 240 bp

>AT216420 NZ_CP113044:c190656-190486 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA