Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicA-HicB
Location 1950397..1951019 Replicon chromosome
Accession NZ_CP083442
Organism Pseudomonas chlororaphis strain L5734

Toxin (Protein)


Gene name hicA Uniprot ID A0A2N8AR08
Locus tag K9V45_RS08915 Protein ID WP_007929414.1
Coordinates 1950397..1950579 (+) Length 61 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A2N8AQX2
Locus tag K9V45_RS08920 Protein ID WP_063429381.1
Coordinates 1950612..1951019 (+) Length 136 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K9V45_RS08885 (K9V45_08885) 1945870..1946808 - 939 WP_226305106.1 hypothetical protein -
K9V45_RS08890 (K9V45_08890) 1946790..1947251 - 462 WP_226305107.1 hypothetical protein -
K9V45_RS08895 (K9V45_08895) 1947300..1947482 + 183 WP_226305617.1 AlpA family phage regulatory protein -
K9V45_RS08900 (K9V45_08900) 1947662..1948132 - 471 WP_226305108.1 AbfB domain-containing protein -
K9V45_RS08905 (K9V45_08905) 1948537..1948839 - 303 WP_226305109.1 hypothetical protein -
K9V45_RS08910 (K9V45_08910) 1948867..1949736 - 870 WP_226305110.1 Arm DNA-binding domain-containing protein -
K9V45_RS08915 (K9V45_08915) 1950397..1950579 + 183 WP_007929414.1 type II toxin-antitoxin system HicA family toxin Toxin
K9V45_RS08920 (K9V45_08920) 1950612..1951019 + 408 WP_063429381.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
K9V45_RS08925 (K9V45_08925) 1951264..1951731 + 468 WP_226305111.1 GAF domain-containing protein -
K9V45_RS08930 (K9V45_08930) 1951861..1952775 + 915 WP_226305112.1 SGNH/GDSL hydrolase family protein -
K9V45_RS08935 (K9V45_08935) 1952809..1954587 - 1779 WP_226305113.1 GGDEF and EAL domain-containing protein -
K9V45_RS08940 (K9V45_08940) 1954823..1955716 - 894 WP_196248185.1 LysR family transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 1943680..1955716 12036


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(6-57)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(3-128)


Sequences

Toxin        


Download         Length: 61 a.a.        Molecular weight: 6850.15 Da        Isoelectric Point: 11.6709

>T216419 WP_007929414.1 NZ_CP083442:1950397-1950579 [Pseudomonas chlororaphis]
VNSRYLIGQIVADGWYLVRVRGSHHHFRHPSKPGLVTVPHPKKDLLRKTAISILKQALLM

Download         Length: 183 bp

>T216419 NZ_CP113044:c92870-92694 [Staphylococcus aureus]
TTGGATAGATGGTGGCTATCTGAGTATAAGGAGGTGGTGCCTATGGTGGCATTACTGAAATCTTTAGAAAGGAGACGCCT
AATGATTACAATTAGTACCATGTTGCAGTTTGGTTTATTCCTTATTGCATTGATAGGTCTAGTAATCAAGCTTATTGAAT
TAAGCAATAAAAAATAA

Antitoxin


Download         Length: 136 a.a.        Molecular weight: 14604.60 Da        Isoelectric Point: 4.3638

>AT216419 WP_063429381.1 NZ_CP083442:1950612-1951019 [Pseudomonas chlororaphis]
MLYPIAISMGDDEHAWGVEVPDIPGCFSAGDDLDDAMAMAREAIEGHFEILAEDGAAIPPANKVTLHAANPQYAGCTWAL
IDIDVTKYLGKAQKLNITLPGYLLNRIDEYVLHHPEAKSRSGFLASAALKVLQQD

Download         Length: 408 bp

>AT216419 NZ_CP113044:92571-92626 [Staphylococcus aureus]
AAAAAGGGCAACATGCGCAAACATGTTACCCTAATGAGCCCGTTAAAAAGACGGTG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2N8AR08

Antitoxin

Source ID Structure
AlphaFold DB A0A2N8AQX2

References