Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relB-yefM/YoeB-YefM
Location 147895..148403 Replicon chromosome
Accession NZ_CP083439
Organism Clavibacter sp. A6099

Toxin (Protein)


Gene name relB Uniprot ID A0A399NVB9
Locus tag KYT88_RS00730 Protein ID WP_043582935.1
Coordinates 147895..148155 (-) Length 87 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID A0A399NV93
Locus tag KYT88_RS00735 Protein ID WP_043582937.1
Coordinates 148152..148403 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KYT88_RS00695 (KYT88_00695) 143076..143933 - 858 WP_043582921.1 3-hydroxyacyl-CoA dehydrogenase -
KYT88_RS00700 (KYT88_00700) 144138..144995 + 858 WP_043582923.1 phosphatase PAP2 family protein -
KYT88_RS00705 (KYT88_00705) 145024..145263 + 240 WP_043582925.1 hypothetical protein -
KYT88_RS00710 (KYT88_00710) 145303..145725 + 423 WP_043582927.1 alternative ribosome rescue aminoacyl-tRNA hydrolase ArfB -
KYT88_RS00715 (KYT88_00715) 145753..146802 - 1050 WP_043582929.1 LLM class flavin-dependent oxidoreductase -
KYT88_RS00720 (KYT88_00720) 146938..147279 + 342 WP_043582931.1 DUF2200 domain-containing protein -
KYT88_RS00725 (KYT88_00725) 147481..147786 + 306 WP_200871128.1 hypothetical protein -
KYT88_RS00730 (KYT88_00730) 147895..148155 - 261 WP_043582935.1 Txe/YoeB family addiction module toxin Toxin
KYT88_RS00735 (KYT88_00735) 148152..148403 - 252 WP_043582937.1 type II toxin-antitoxin system prevent-host-death family antitoxin Antitoxin
KYT88_RS00740 (KYT88_00740) 148467..148694 - 228 WP_043582939.1 DUF2283 domain-containing protein -
KYT88_RS00745 (KYT88_00745) 148694..149269 - 576 WP_043582941.1 TetR family transcriptional regulator -
KYT88_RS00750 (KYT88_00750) 149366..150487 + 1122 WP_043582943.1 zinc-binding alcohol dehydrogenase family protein -
KYT88_RS00755 (KYT88_00755) 150520..151710 - 1191 WP_147362324.1 histidine kinase -
KYT88_RS00760 (KYT88_00760) 151957..152649 + 693 WP_051629158.1 response regulator transcription factor -
KYT88_RS00765 (KYT88_00765) 152757..153056 + 300 WP_043582947.1 rhodanese-like domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(7-86)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 87 a.a.        Molecular weight: 10109.42 Da        Isoelectric Point: 7.3158

>T216415 WP_043582935.1 NZ_CP083439:c148155-147895 [Clavibacter sp. A6099]
VSRQLAFDADGWDDYTYWQGQDRKTLKRINALIRDVMRDPFEGPGKPEPLKHILSGAWSRRIDEANRLVYLVTDTHIVIL
QARDHY

Download         Length: 261 bp

>T216415 NZ_CP113042:c589062-588796 [Staphylococcus aureus]
ATGAGCAATTACACGGTTAAGATTAAAAATTCAGCGAAATCAGATTTAAAGAAAATAAAACATTCTTATTTAAAGAAGTC
ATTTTTAGAAATTGTTGAGACTTTAAAAAATGATCCGTATAAAATAACACAATCTTTTGAAAAATTAGAGCCTAAATATT
TAGAGCGATATTCAAGAAGAATTAACCATCAGCACAGGGTCGTCTATACCGTAGATGATCGAAATAAAGAAGTATTAATA
CTATCGGCATGGTCACATTATGATTAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9206.28 Da        Isoelectric Point: 5.0859

>AT216415 WP_043582937.1 NZ_CP083439:c148403-148152 [Clavibacter sp. A6099]
MSAITASDARRNLFGLIQQVNDDHSPVEVVSKHGNAVLISKDDFDAITETAYLLRNAKGAQRLLGALERARRGEFETHEL
IDE

Download         Length: 252 bp

>AT216415 NZ_CP113042:c589319-589062 [Staphylococcus aureus]
ATGATTATCACTAGCCCTACAGAAGCGAGAAAAGATTTTTATCAATTACTAAAAAATGTTAATAATAATCACGAACCAAT
TTATATTAGTGGCAATAATGCCGAAAATAATGCTGTGATTATAGGTTTAGAAGATTGGAAAAGTATACAAGAGACAATAT
ATCTTGAATCTACTGGTACAATGGACAAAGTAAGAGAAAGAGAAAAAGATAATAGTGGTACAACAAATATAGATGATATT
GATTGGGATAATCTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A399NVB9

Antitoxin

Source ID Structure
AlphaFold DB A0A399NV93

References