Detailed information of TA system TA216414

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): spoIISABC/SpoIISA-SpoIISB
• Location: 1246636..1247553
• Replicon: chromosome
• Accession: NZ_CP083436
• Organism: Bacillus velezensis strain HC-8

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: I2HQ15
• Locus tag: K9864_RS06570
• Protein ID: WP_007407256.1
• Coordinates: 1246807..1247553 (-)
• Length: 249 a.a.

Antitoxin (Protein)

• Gene name: spoIISB
• Uniprot ID: I2C3Z8
• Locus tag: K9864_RS06565
• Protein ID: WP_014417527.1
• Coordinates: 1246636..1246806 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K9864_RS06515 (1241842)	1241842..1242354	+	513	WP_012117362.1	sigma-70 family RNA polymerase sigma factor	-
K9864_RS06520 (1242467)	1242467..1243018	+	552	Protein_1223	terminase	-
K9864_RS06525 (1243129)	1243129..1243491	+	363	WP_014721043.1	hypothetical protein	-
K9864_RS06530 (1243504)	1243504..1243875	+	372	WP_014417525.1	XkdW family protein	-
K9864_RS06535 (1243880)	1243880..1244077	+	198	WP_007610833.1	XkdX family protein	-
K9864_RS06540 (1244134)	1244134..1244895	+	762	WP_014417526.1	hypothetical protein	-
K9864_RS06545 (1244947)	1244947..1245210	+	264	WP_003154815.1	hemolysin XhlA family protein	-
K9864_RS06550 (1245224)	1245224..1245487	+	264	WP_003154813.1	phage holin	-
K9864_RS06555 (1245501)	1245501..1246379	+	879	WP_007407257.1	N-acetylmuramoyl-L-alanine amidase	-
K9864_RS06560 (1246414)	1246414..1246539	-	126	WP_003154809.1	hypothetical protein	-
K9864_RS06565 (1246636)	1246636..1246806	-	171	WP_014417527.1	type II toxin-antitoxin system SpoIISB family antitoxin	Antitoxin
K9864_RS06570 (1246807)	1246807..1247553	-	747	WP_007407256.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
K9864_RS06575 (1247657)	1247657..1248655	-	999	WP_003154805.1	inorganic phosphate transporter	-
K9864_RS06580 (1248668)	1248668..1249285	-	618	WP_003154804.1	DUF47 domain-containing protein	-
K9864_RS06585 (1249571)	1249571..1250887	-	1317	WP_007610842.1	amino acid permease	-
K9864_RS06590 (1251209)	1251209..1252159	+	951	WP_014417529.1	ring-cleaving dioxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1232783..1255769	22986

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (3-239)

Antitoxin

• Antitoxin SpoIISB (1-56)

Sequences

Toxin

Protein

• Length: 249 a.a.
• Molecular weight: 29077.57 Da
• Isoelectric Point: 4.7755

>T216414 WP_007407256.1 NZ_CP083436:c1247553-1246807 [Bacillus velezensis]
MLLFFQIMVWTMAAALILYVYASWRYEAKVKEKMFAIRKTWYLLFVLGSMVYWTYDPESLFAAWRQYLIVAVCFALIDAF
IFLSAYIKKLAGNELETDTREILEENNEMLHSYLEKLKTYQYLLKNEPIHVYYGSTEAYAEGISRLLAAYAEKMNVTASL
CDYSAQSDKDRLTEHMPDAADVQSRLNRKDVYYDQKGRLVLIPFTVQNRHYVIKLTSENLLTEFDYLLFTSLTSIYDLML
PIEEEGDG

Nucleotide

• Length: 747 bp

>T216414 NZ_CP113042:c557480-557373 [Staphylococcus aureus]
ATGTTCAATTTATTAATTAACATCATGACTTCAGCTATAAGCGGCTGTCTTGTTGCGTTTTTTGCACATTGGTTACGAAC
GCGCAACAATAAAAAAGGTGACAAATAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6676.78 Da
• Isoelectric Point: 10.9951

>AT216414 WP_014417527.1 NZ_CP083436:c1246806-1246636 [Bacillus velezensis]
MEQAFQNGQPEKRAKPFKMFKKRSMTSIASYQVSPHTARIFKENERLIDKYKQKKA

Nucleotide

• Length: 171 bp

>AT216414 NZ_CP113042:557296-557356 [Staphylococcus aureus]
TACATAATAAATTGAACATCTAAATACACCAAATCCCCTCACTACTGCCATAGTGAGGGGA

Structures

Toxin

• AlphaFold DB: I2HQ15

Antitoxin

• AlphaFold DB: I2C3Z8