Detailed information of TA system TA216413

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 468199..468836
• Replicon: chromosome
• Accession: NZ_CP083436
• Organism: Bacillus velezensis strain HC-8

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G4NU33
• Locus tag: K9864_RS02410
• Protein ID: WP_003156187.1
• Coordinates: 468486..468836 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: I2HMS5
• Locus tag: K9864_RS02405
• Protein ID: WP_003156188.1
• Coordinates: 468199..468480 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K9864_RS02385 (464564)	464564..465163	-	600	WP_003156193.1	rhomboid family intramembrane serine protease	-
K9864_RS02390 (465256)	465256..465621	+	366	WP_014304402.1	holo-ACP synthase	-
K9864_RS02395 (465786)	465786..466793	+	1008	WP_007410230.1	outer membrane lipoprotein carrier protein LolA	-
K9864_RS02400 (466910)	466910..468079	+	1170	WP_014416966.1	alanine racemase	-
K9864_RS02405 (468199)	468199..468480	+	282	WP_003156188.1	type II toxin-antitoxin system antitoxin EndoAI	Antitoxin
K9864_RS02410 (468486)	468486..468836	+	351	WP_003156187.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
K9864_RS02415 (468954)	468954..469775	+	822	WP_003156182.1	STAS domain-containing protein	-
K9864_RS02420 (469780)	469780..470145	+	366	WP_007609584.1	RsbT antagonist protein RsbS	-
K9864_RS02425 (470148)	470148..470549	+	402	WP_003156178.1	anti-sigma regulatory factor	-
K9864_RS02430 (470561)	470561..471568	+	1008	WP_007609589.1	PP2C family protein-serine/threonine phosphatase	-
K9864_RS02435 (471632)	471632..471961	+	330	WP_003156176.1	anti-sigma factor antagonist RsbV	-
K9864_RS02440 (471958)	471958..472440	+	483	WP_007609591.1	anti-sigma B factor RsbW	-
K9864_RS02445 (472406)	472406..473194	+	789	WP_003156171.1	RNA polymerase sigma factor SigB	-
K9864_RS02450 (473194)	473194..473796	+	603	WP_007410234.1	PP2C family serine/threonine-protein phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12977.98 Da
• Isoelectric Point: 4.8781

>T216413 WP_003156187.1 NZ_CP083436:468486-468836 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Nucleotide

• Length: 351 bp

>T216413 NZ_CP113042:c532214-531948 [Staphylococcus aureus]
ATGGCTAGGTTAAATATTACGTTTTCGCCTCAAGCCTTTGAAGATTATAAGTATTTTCAGCAGAACGATAAAAAAATGGT
GAAGAAGATTAATGAGTTACTTAAAAGTATTGACAGAAATGGTGCATTGGAAGGTATAGGTAAGCCTGAAAAGTTAAAAT
CGAATCTGACTGGGTATTATAGTAGACGTATCAATCACGAACATAGATTGGTTTATACAGTAGATGACAATCATATAAAA
ATAGCATCATGTAAATACCATTATTAA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10562.92 Da
• Isoelectric Point: 6.4706

>AT216413 WP_003156188.1 NZ_CP083436:468199-468480 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Nucleotide

• Length: 282 bp

>AT216413 NZ_CP113042:c532465-532214 [Staphylococcus aureus]
ATGATTATTAAAAATTATTCATACGCTCGACAGAATTTAAAGGCACTTATGACAAAAGTAAATGATGATAGTGTTATGGT
AACTGTAACATCTACTGATGATAAAAACGTAGTAATCATGTCAGAATCAGATTATAACTCCATGATGGAAACACTTTACC
TCCAACAGAACCCAAATAATGCTGAACACTTAGCTCAATCAATTGCAGATCTAGAACGTGGGAAAACTATAACGAAAGAT
ATAGATGTATAA

Structures

Toxin

• PDB: 4MDX
• PDB: 4ME7
• PDB: 1NE8

Antitoxin

• AlphaFold DB: I2HMS5