Detailed information of TA system TA216411

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2585249..2585778
• Replicon: chromosome
• Accession: NZ_CP083434
• Organism: Staphylococcus aureus strain DC.RB_015

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: LAE57_RS12450
• Protein ID: WP_000621175.1
• Coordinates: 2585416..2585778 (+)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: LAE57_RS12445
• Protein ID: WP_000948331.1
• Coordinates: 2585249..2585419 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LAE57_RS12415 (2580285)	2580285..2580845	+	561	WP_001092405.1	K(+)-transporting ATPase subunit C	-
LAE57_RS12420 (2581054)	2581054..2581533	+	480	WP_001287083.1	hypothetical protein	-
LAE57_RS12425 (2581526)	2581526..2583109	+	1584	WP_217657974.1	PH domain-containing protein	-
LAE57_RS12430 (2583096)	2583096..2583587	+	492	WP_001205915.1	PH domain-containing protein	-
LAE57_RS12435 (2583591)	2583591..2583950	+	360	WP_000581194.1	holo-ACP synthase	-
LAE57_RS12440 (2584016)	2584016..2585164	+	1149	WP_224056908.1	alanine racemase	-
LAE57_RS12445 (2585249)	2585249..2585419	+	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
LAE57_RS12450 (2585416)	2585416..2585778	+	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
LAE57_RS12455 (2586127)	2586127..2587128	+	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
LAE57_RS12460 (2587247)	2587247..2587573	+	327	WP_001052491.1	anti-sigma factor antagonist	-
LAE57_RS12465 (2587575)	2587575..2588054	+	480	WP_001190825.1	anti-sigma B factor RsbW	-
LAE57_RS12470 (2588029)	2588029..2588799	+	771	WP_001041107.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T216411 WP_000621175.1 NZ_CP083434:2585416-2585778 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T216411 NZ_CP113042:c210093-209884 [Staphylococcus aureus]
ATGATACATCAAAATACGATTTACACAGCGGGAATTGAAACAGAAGAACAAGTAAGTCAATTGACAGAACGCATTTCAAA
TATGATAGGTGTTCATCAAGTGAATATTAATATAATAGATGGTCAAGTAACTGTATCGTATGAGACACCAGCAAATTTGA
ATAGTATTGAAAAAGAAATCTATGATGAAGGATACAAAATTGTATTTTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT216411 WP_000948331.1 NZ_CP083434:2585249-2585419 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT216411 NZ_CP113042:c210398-210105 [Staphylococcus aureus]
ATGACTGAACAAGATAATGCACATCATTCTGAACAAATAAAAACGAATCTTAAATCACGTTTAAATCGAATTGAAGGACA
AGTGAGAGCGATTAATCGCATGATTGAAGAAGATGTCTATTGTGATGATGTCCTTACGCAAATAAGAGCGACACGTTCGG
CGTTAAACAGTGTTGCGATAAAGTTATTAGAACAACATATGAAAAGTTGTATTATGAATAAAGTTAATCAAGGTGCTCAG
GAAGAGGCAATGGAAGAGTTATTAGTGACTTTTCAAAAATTGATTAAAGACTAA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1