Detailed information of TA system TA216410

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsdAT/-
• Location: 2565524..2566038
• Replicon: chromosome
• Accession: NZ_CP083434
• Organism: Staphylococcus aureus strain DC.RB_015

Toxin (Protein)

• Gene name: TsdT
• Uniprot ID: T1YA99
• Locus tag: LAE57_RS12360
• Protein ID: WP_000581792.1
• Coordinates: 2565829..2566038 (+)
• Length: 70 a.a.

Antitoxin (Protein)

• Gene name: TsdA
• Uniprot ID: A0A0D1GBN9
• Locus tag: LAE57_RS12355
• Protein ID: WP_000138934.1
• Coordinates: 2565524..2565817 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LAE57_RS12335 (2560785)	2560785..2561426	+	642	WP_000483147.1	thiamine phosphate synthase	-
LAE57_RS12340 (2561512)	2561512..2562384	+	873	WP_000725802.1	membrane protein insertase YidC	-
LAE57_RS12345 (2563199)	2563199..2563846	-	648	WP_001187610.1	HD domain-containing protein	-
LAE57_RS12350 (2563874)	2563874..2565358	-	1485	WP_057504585.1	cardiolipin synthase	-
LAE57_RS12355 (2565524)	2565524..2565817	+	294	WP_000138934.1	copper-sensing transcriptional repressor CsoR	Antitoxin
LAE57_RS12360 (2565829)	2565829..2566038	+	210	WP_000581792.1	heavy metal-associated domain-containing protein	Toxin
LAE57_RS12365 (2566166)	2566166..2566303	+	138	WP_000828354.1	Lmo0850 family protein	-
LAE57_RS12370 (2566567)	2566567..2567769	-	1203	WP_001109939.1	FtsW/RodA/SpoVE family cell cycle protein	-
LAE57_RS12375 (2568087)	2568087..2569157	+	1071	WP_000159641.1	D-alanine--D-alanine ligase	-
LAE57_RS12380 (2569172)	2569172..2570530	+	1359	WP_000611468.1	UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Heavy metal-associated domain, HMA (23-66)

Antitoxin

• Metal-sensitive transcriptional repressor (3-95)

Sequences

Toxin

Protein

• Length: 70 a.a.
• Molecular weight: 7843.77 Da
• Isoelectric Point: 4.1008

>T216410 WP_000581792.1 NZ_CP083434:2565829-2566038 [Staphylococcus aureus]
MIHQNTIYTAGIETEEQVSQLTERISNMIGVHQVNINIIDGQVTVSYETPANLNSIEKEIYDEGYKIVF

Nucleotide

• Length: 210 bp

>T216410 NZ_CP113042:c190490-190128 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAGTTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCACCAGAAAAATTAG

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11185.77 Da
• Isoelectric Point: 6.2243

>AT216410 WP_000138934.1 NZ_CP083434:2565524-2565817 [Staphylococcus aureus]
MTEQDNAHHSEQIKTNLKSRLNRIEGQVRAINRMIEEDVYCDDVLTQIRATRSALNSVATKLLEQHMKSCIMNKVNQGAQ
EEAMEELLVTFQKLIKD

Nucleotide

• Length: 294 bp

>AT216410 NZ_CP113042:c190657-190487 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Toxin

• PDB: 6FF1

Antitoxin

• AlphaFold DB: A0A0D1GBN9