Detailed information of TA system TA216402

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Xre-MNT/HTH_26(antitoxin)
• Location: 2022512..2023620
• Replicon: chromosome
• Accession: NZ_CP083434
• Organism: Staphylococcus aureus strain DC.RB_015

Toxin (Protein)

• Gene name: MNTss
• Uniprot ID: R3JIN1
• Locus tag: LAE57_RS09595
• Protein ID: WP_000233000.1
• Coordinates: 2022751..2023620 (+)
• Length: 290 a.a.

Antitoxin (Protein)

• Gene name: Xress
• Uniprot ID: -
• Locus tag: LAE57_RS09590
• Protein ID: WP_000205227.1
• Coordinates: 2022512..2022736 (+)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LAE57_RS09560 (2018101)	2018101..2018271	+	171	WP_000713595.1	hypothetical protein	-
LAE57_RS09565 (2018285)	2018285..2018903	+	619	Protein_1887	recombinase family protein	-
LAE57_RS09570 (2018903)	2018903..2019580	+	678	Protein_1888	DNA topoisomerase	-
LAE57_RS09575 (2020062)	2020062..2020388	+	327	WP_000392725.1	hypothetical protein	-
LAE57_RS09580 (2020389)	2020389..2020805	+	417	WP_000323438.1	recombinase	-
LAE57_RS09585 (2020825)	2020825..2022369	+	1545	WP_002390960.1	recombinase family protein	-
LAE57_RS09590 (2022512)	2022512..2022736	+	225	WP_000205227.1	helix-turn-helix transcriptional regulator	Antitoxin
LAE57_RS09595 (2022751)	2022751..2023620	+	870	WP_000233000.1	nucleotidyltransferase domain-containing protein	Toxin
LAE57_RS09600 (2023601)	2023601..2024335	+	735	WP_000662263.1	class I SAM-dependent methyltransferase	-
LAE57_RS09605 (2024368)	2024368..2025276	+	909	WP_001255866.1	aminoglycoside nucleotidyltransferase ANT(6)-Ia	-
LAE57_RS09610 (2025273)	2025273..2025753	+	481	Protein_1896	GNAT family N-acetyltransferase	-
LAE57_RS09615 (2025846)	2025846..2026640	+	795	WP_001096887.1	aminoglycoside O-phosphotransferase APH(3')-IIIa	-
LAE57_RS09620 (2027182)	2027182..2027265	+	84	WP_001814874.1	23S rRNA methyltransferase attenuation leader peptide	-
LAE57_RS09625 (2027390)	2027390..2028127	+	738	WP_001038790.1	23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B)	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	ant(6)-Ia / aph(3')-III / erm(B)	-	2017098..2033043	15945
	-	flank	IS/Tn	ant(6)-Ia / aph(3')-III / erm(B)	-	2024368..2033043	8675

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polymerase, nucleotidyl transferase domain (12-111)

Antitoxin

• Cro/C1-type helix-turn-helix domain (4-57)

Sequences

Toxin

Protein

• Length: 290 a.a.
• Molecular weight: 32926.57 Da
• Isoelectric Point: 4.8781

>T216402 WP_000233000.1 NZ_CP083434:2022751-2023620 [Staphylococcus aureus]
MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIYYNSDSFDLTAINQIATELDDKNRNNLVVPPGAWGDW
INGGGWLFINGYHVDLILRDIKRVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
PTALKKSLMNFFIFEAEFSLMFVKANAGVEDKYYIAGHVFRIISCLNQVLFACNNAYCINEKKAIKLLETFEYKPEKYAE
RVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINNFLNEESSDERKQI

Nucleotide

• Length: 870 bp

>T216402 NZ_CP113039:c523613-523509 [Staphylococcus aureus]
TTGTTACTCCTAGAAAGGACTAGCATGTCTGATTTTGAAATGCTGATGGTTGTATTAACAATCATTGGTTTAGTATTGAT
TAGTACTCAAGACCATAAAAAATAA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8365.68 Da
• Isoelectric Point: 4.5510

>AT216402 WP_000205227.1 NZ_CP083434:2022512-2022736 [Staphylococcus aureus]
MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLDELKAIADVLGVKFVQAFILPDGEEIKISNE

Nucleotide

• Length: 225 bp

>AT216402 NZ_CP113039:523416-523454 [Staphylococcus aureus]
AACATGTTACCCTAATGAGCCCGTTAAAAAGACGGTGAC

Structures

Toxin

• AlphaFold DB: R3JIN1