Detailed information of TA system TA216401

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsaAT/-
• Location: 1033024..1033501
• Replicon: chromosome
• Accession: NZ_CP083434
• Organism: Staphylococcus aureus strain DC.RB_015

Toxin (Protein)

• Gene name: TsaT
• Uniprot ID: T1Y7S2
• Locus tag: LAE57_RS04975
• Protein ID: WP_000052076.1
• Coordinates: 1033313..1033501 (+)
• Length: 63 a.a.

Antitoxin (Protein)

• Gene name: TsaA
• Uniprot ID: T1Y6P8
• Locus tag: LAE57_RS04970
• Protein ID: WP_001059690.1
• Coordinates: 1033024..1033284 (+)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LAE57_RS04935 (1028416)	1028416..1029132	-	717	WP_000150029.1	type I 3-dehydroquinate dehydratase	-
LAE57_RS04940 (1029280)	1029280..1029702	+	423	WP_000974455.1	organic hydroperoxide resistance protein	-
LAE57_RS04945 (1030101)	1030101..1030595	+	495	WP_000569881.1	GNAT family N-acetyltransferase	-
LAE57_RS04950 (1030751)	1030751..1031368	+	618	WP_000255554.1	LysE/ArgO family amino acid transporter	-
LAE57_RS04955 (1031441)	1031441..1032022	-	582	WP_001140697.1	histidine phosphatase family protein	-
LAE57_RS04960 (1032247)	1032247..1032483	+	237	WP_000368687.1	hypothetical protein	-
LAE57_RS04965 (1032480)	1032480..1032683	-	204	WP_000145163.1	sterile alpha motif-like domain-containing protein	-
LAE57_RS04970 (1033024)	1033024..1033284	+	261	WP_001059690.1	hypothetical protein	Antitoxin
LAE57_RS04975 (1033313)	1033313..1033501	+	189	WP_000052076.1	hypothetical protein	Toxin
LAE57_RS04980 (1033689)	1033689..1034258	+	570	WP_001106657.1	lipoprotein N-acylation protein LnsA	-
LAE57_RS04985 (1034346)	1034346..1034630	+	285	WP_001004397.1	hypothetical protein	-
LAE57_RS04990 (1034692)	1034692..1034910	+	219	WP_000005888.1	hypothetical protein	-
LAE57_RS04995 (1035409)	1035409..1035609	-	201	WP_001059082.1	cold-shock protein	-
LAE57_RS05000 (1035966)	1035966..1036652	-	687	WP_001823812.1	thermonuclease family protein	-
LAE57_RS05005 (1036993)	1036993..1037463	-	471	WP_000824462.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	seb / selq / selk / vWbp	1005472..1051282	45810

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Metal-sensitive transcriptional repressor (14-92)

Sequences

Toxin

Protein

• Length: 63 a.a.
• Molecular weight: 7142.82 Da
• Isoelectric Point: 9.9971

>T216401 WP_000052076.1 NZ_CP083434:1033313-1033501 [Staphylococcus aureus]
MSLHFAILFWLALIFLVAATFILVLMKKTGKESKKESYLSFTVILYIFGFAILIYTFIFGVL

Nucleotide

• Length: 189 bp

>T216401 NZ_CP113039:c466217-466008 [Staphylococcus aureus]
ATGATACATCAAAATACGATTTACACAGCGGGAATTGAAACAGAAGAACAAGTAAGTCAATTGACAGAACGCATTTCAAA
TATGATAGGTGTTCATCAAGTGAATATTAATATAATAGATGGTCAAGTAACTGTATCGTATGAGACACCAGCAAATTTGA
ATAGTATTGAAAAAGAAATCTATGATGAAGGATACAAAATTGTATTTTAG

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9940.09 Da
• Isoelectric Point: 4.0624

>AT216401 WP_001059690.1 NZ_CP083434:1033024-1033284 [Staphylococcus aureus]
MNNILLNAINIVITTTFVIFNILITYNKDLDDLCWLLPGIIICGVILIVSFTIAMITKNWLSEILFFINIVLVLYYIYPI
FYSFIG

Nucleotide

• Length: 261 bp

>AT216401 NZ_CP113039:c466522-466229 [Staphylococcus aureus]
ATGACTGAACAAGATAATGCACATCATTCTGAACAAATAAAAACGAATCTTAAATCACGTTTAAATCGAATTGAAGGACA
AGTGAGAGCGATTAATCGCATGATTGAAGAAGATGTCTATTGTGATGATGTCCTTACGCAAATAAGAGCGACACGTTCGG
CGTTAAACAGTGTTGCGATAAAGTTATTAGAACAACATATGAAAAGTTGTATTATGAATAAAGTTAATCAAGGTGCTCAG
GAAGAGGCAATGGAAGAGTTATTAGTGACTTTTCAAAAATTGATTAAAGACTAA

Structures

Toxin

• AlphaFold DB: A0A0E0VP18

Antitoxin

• AlphaFold DB: A0A0E0VN56