Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3040278..3040932 Replicon chromosome
Accession NZ_CP083410
Organism Escherichia coli strain RP437

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag LAJ62_RS14830 Protein ID WP_000244777.1
Coordinates 3040278..3040685 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag LAJ62_RS14835 Protein ID WP_000354046.1
Coordinates 3040666..3040932 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LAJ62_RS14810 (3036235) 3036235..3037968 - 1734 WP_000813200.1 single-stranded-DNA-specific exonuclease RecJ -
LAJ62_RS14815 (3037974) 3037974..3038684 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
LAJ62_RS14820 (3038709) 3038709..3039605 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
LAJ62_RS14825 (3039717) 3039717..3040238 + 522 WP_001055874.1 flavodoxin FldB -
LAJ62_RS14830 (3040278) 3040278..3040685 - 408 WP_000244777.1 protein YgfX Toxin
LAJ62_RS14835 (3040666) 3040666..3040932 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
LAJ62_RS14840 (3041175) 3041175..3042155 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
LAJ62_RS14845 (3042351) 3042351..3043010 - 660 WP_000250274.1 hemolysin III family protein -
LAJ62_RS14850 (3043174) 3043174..3043485 - 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
LAJ62_RS14855 (3043530) 3043530..3044963 + 1434 WP_001336277.1 6-phospho-beta-glucosidase BglA -
LAJ62_RS14860 (3045020) 3045020..3045763 - 744 WP_000951964.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T216385 WP_000244777.1 NZ_CP083410:c3040685-3040278 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T216385 NZ_CP113032:c2581806-2581540 [Staphylococcus aureus]
ATGGCTAGGTTAAATATTACGTTTTCGCCTCAAGCCTTTGAAGATTATAAGTATTTTCAGCAGAACGATAAAAAAATGGT
GAAGAAGATTAATGAGTTACTTAAAAGTATTGACAGAAATGGTGCATTGGAAGGTATAGGTAAGCCTGAAAAGTTAAAAT
CGAATCTGACTGGGTATTATAGTAGACGTATCAATCACGAACATAGATTGGTTTATACAGTAGATGACAATCATATAAAA
ATAGCATCATGTAAATACCATTATTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT216385 WP_000354046.1 NZ_CP083410:c3040932-3040666 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT216385 NZ_CP113032:c2582057-2581806 [Staphylococcus aureus]
ATGATTATTAAAAATTATTCATACGCTCGACAGAATTTAAAGGCACTTATGACAAAAGTAAATGATGATAGTGATATGGT
AACTGTAACATCTACTGATGATAAAAACGTAGTAATCATGTCAGAATCAGATTATAACTCCATGATGGAAACACTTTACC
TCCAACAGAACCCAAATAATGCTGAACACTTAGCTCAATCAATTGCAGATCTAGAACGTGGGAAAACTATAACGAAAGAT
ATAGATGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References