216383

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yfjZ-ypjF/CbtA-CbeA
Location 2776978..2777645 Replicon chromosome
Accession NZ_CP083410
Organism Escherichia coli strain RP437

Toxin (Protein)


Gene name ypjF Uniprot ID Q46953
Locus tag LAJ62_RS13615 Protein ID WP_001094400.1
Coordinates 2777316..2777645 (+) Length 110 a.a.

Antitoxin (Protein)


Gene name yfjZ Uniprot ID P52141
Locus tag LAJ62_RS13610 Protein ID WP_000072690.1
Coordinates 2776978..2777295 (+) Length 106 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LAJ62_RS13580 (2772030) 2772030..2773039 - 1010 Protein_2658 arsenic transporter -
LAJ62_RS13585 (2773181) 2773181..2774884 + 1704 WP_001698958.1 protein YfjW -
LAJ62_RS13590 (2775456) 2775456..2775679 + 224 Protein_2660 DUF905 domain-containing protein -
LAJ62_RS13595 (2775782) 2775782..2776240 + 459 WP_000211841.1 antirestriction protein -
LAJ62_RS13600 (2776249) 2776249..2776731 + 483 WP_001407480.1 RadC family protein -
LAJ62_RS13605 (2776740) 2776740..2776940 + 201 WP_001395452.1 DUF987 domain-containing protein -
LAJ62_RS13610 (2776978) 2776978..2777295 + 318 WP_000072690.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
LAJ62_RS13615 (2777316) 2777316..2777645 + 330 WP_001094400.1 type IV toxin-antitoxin system toxin YpjF Toxin
LAJ62_RS13620 (2778009) 2778009..2782589 - 4581 WP_010723187.1 adhesin-like autotransporter YpjA/EhaD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-108)

Antitoxin

Antitoxin CbeA

(5-104)


Sequences

Toxin        


Download         Length: 110 a.a.        Molecular weight: 12308.21 Da        Isoelectric Point: 7.2761

>T216383 WP_001094400.1 NZ_CP083410:2777316-2777645 [Escherichia coli]
MNTLPATISQAAKPCLSPVAVWQMLLTRLLEQHYGLTLNDTPFSDETVIKEHIDAGITLADAVNFLVEKYELVRIDHRGF
SWQQQSPYISVVDILRARRSTGLLKTNVK

Download         Length: 330 bp

>T216383 NZ_CP113032:c2259481-2259272 [Staphylococcus aureus]
ATGATACATCAAAATACGATTTACACAGCGGGAATTGAAACAGAAGAACAAGTAAGTCAATTGACAGAACGCATTTCAAA
TATGATAGGTGTTCATCAAGTGAATATTAATATAATAGATGGTCAAGTAACTGTATCGTATGAGACACCAGCAAATTTGA
ATAGTATTGAAAAAGAAATCTATGATGAAGGATACAAAATTGTATTTTAG

Antitoxin


Download         Length: 106 a.a.        Molecular weight: 11737.27 Da        Isoelectric Point: 6.4630

>AT216383 WP_000072690.1 NZ_CP083410:2776978-2777295 [Escherichia coli]
MSNTTWGLQRDITPRLGARLVQEGNQLHYLADRASITGKFSDAECPKLDVVFPHFISQIESMLTTGELNPRHAQCVTLYH
NGFTCEADTLGSCGYVYIAVYPTQR

Download         Length: 318 bp

>AT216383 NZ_CP113032:c2259786-2259493 [Staphylococcus aureus]
ATGACTGAACAAGATAATGCACATCATTCTGAACAAATAAAAACGAATCTTAAATCACGTTTAAATCGAATTGAAGGACA
AGTGAGAGCGATTAATCGCATGATTGAAGAAGATGTCTATTGTGATGATGTCCTTACGCAAATAAGAGCGACACGTTCGG
CGTTAAACAGTGTTGCGATAAAGTTATTAGAACAACATATGAAAAGTTGTATTATGAATAAAGTTAATCAAGGTGCTCAG
GAAGAGGCAATGGAAGAGTTATTAGTGACTTTTCAAAAATTGATTAAAGACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A373F4I3

Antitoxin

Source ID Structure
PDB 2EA9
PDB 2JN7
AlphaFold DB P52141

References