Detailed information of TA system TA021638

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4835384..4836219
• Replicon: chromosome
• Accession: NC_008253
• Organism: Escherichia coli 536

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: Q1R2L4
• Locus tag: ECP_RS23515
• Protein ID: WP_001094426.1
• Coordinates: 4835842..4836219 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: Q1R2L5
• Locus tag: ECP_RS23510
• Protein ID: WP_001285575.1
• Coordinates: 4835384..4835752 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECP_RS23480	4831277..4831957	+	681	WP_001282919.1	WYL domain-containing protein	-
ECP_RS23485	4832105..4832782	+	678	WP_001097301.1	hypothetical protein	-
ECP_RS26030	4832788..4833021	+	234	WP_001278290.1	DUF905 family protein	-
ECP_RS23490	4833111..4833929	+	819	WP_001175158.1	DUF945 domain-containing protein	-
ECP_RS23495	4834020..4834505	+	486	WP_000213697.1	antirestriction protein	-
ECP_RS23500	4834520..4834996	+	477	WP_001298074.1	RadC family protein	-
ECP_RS23505	4835083..4835304	+	222	WP_000692350.1	DUF987 domain-containing protein	-
ECP_RS23510	4835384..4835752	+	369	WP_001285575.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
ECP_RS23515	4835842..4836219	+	378	WP_001094426.1	TA system toxin CbtA family protein	Toxin
ECP_RS23520	4836216..4836704	+	489	WP_000761683.1	hypothetical protein	-
ECP_RS23525	4836716..4836913	+	198	WP_000839272.1	DUF957 domain-containing protein	-
ECP_RS27395	4836998..4837150	+	153	WP_011478345.1	hypothetical protein	-
ECP_RS23530	4838102..4840138	+	2037	WP_000417001.1	hypothetical protein	-
ECP_RS23535	4840537..4840716	-	180	WP_001298063.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	fimB / fimE / fimA / fimI / fimC	4820722..4850267	29545

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14072.99 Da
• Isoelectric Point: 7.3523

>T21638 WP_001094426.1 NC_008253:4835842-4836219 [Escherichia coli 536]
MNTLPDTHIREASHCQSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMARDNYRTVNNITLGKHPGAKQ

Nucleotide

• Length: 378 bp

>T21638 NC_008253:4835842-4836219 [Escherichia coli 536]
ATGAACACATTACCCGATACACACATACGGGAGGCATCGCATTGCCAGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCATTACGGCCTCACACTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTTGAAAAATACGCACTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACTCGTTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCCCGCCGGGCAACCGGCCTGATGGCCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAACATCCGGGGGCGAAACAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13709.52 Da
• Isoelectric Point: 6.6258

>AT21638 WP_001285575.1 NC_008253:4835384-4835752 [Escherichia coli 536]
VSDTLPGTTHPDDNNDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKRLTCEADTLGSCGYVYMAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT21638 NC_008253:4835384-4835752 [Escherichia coli 536]
GTGTCAGACACTCTCCCCGGGACAACGCATCCCGACGATAACAACGACCGCCCCTGGTGGGGGCTACCCTGCACCGTGAC
GCCCTGTTTTGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCAGACCGCGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATGCGTACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAAGGCTGACCTGCGAAGCCGACACCCTCGGCAGTTG
TGGCTACGTTTATATGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A454ABD6

Antitoxin

• AlphaFold DB: A0A454ABF5