Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4583055..4583552 Replicon chromosome
Accession NC_008253
Organism Escherichia coli 536

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag ECP_RS22280 Protein ID WP_001173343.1
Coordinates 4583379..4583552 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID S1NQ59
Locus tag ECP_RS22275 Protein ID WP_001312331.1
Coordinates 4583055..4583351 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECP_RS22255 4579784..4580503 - 720 WP_000611288.1 two-component system response regulator DcuR -
ECP_RS22260 4580500..4582131 - 1632 WP_001216466.1 sensor histidine kinase -
ECP_RS22265 4582312..4582542 + 231 WP_000373291.1 (4Fe-4S)-binding protein -
ECP_RS22270 4582554..4582826 + 273 WP_000405647.1 N-acetyltransferase -
ECP_RS22275 4583055..4583351 + 297 WP_001312331.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
ECP_RS22280 4583379..4583552 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
ECP_RS22285 4583671..4585188 - 1518 WP_000003805.1 lysine--tRNA ligase -
ECP_RS22290 4585425..4586882 - 1458 WP_000856826.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T21637 WP_001173343.1 NC_008253:4583379-4583552 [Escherichia coli 536]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T21637 NC_008253:4583379-4583552 [Escherichia coli 536]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTTCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11440.95 Da        Isoelectric Point: 4.1705

>AT21637 WP_001312331.1 NC_008253:4583055-4583351 [Escherichia coli 536]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLSIMTVDDYFRQFYK

Download         Length: 297 bp

>AT21637 NC_008253:4583055-4583351 [Escherichia coli 536]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAACTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAGCATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
AlphaFold DB S1NQ59

References