Detailed information of TA system TA216369

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 1509283..1509921
• Replicon: chromosome
• Accession: NZ_CP083410
• Organism: Escherichia coli strain RP437

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: LAJ62_RS07420
• Protein ID: WP_000813794.1
• Coordinates: 1509283..1509459 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: LAJ62_RS07425
• Protein ID: WP_001270286.1
• Coordinates: 1509505..1509921 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LAJ62_RS07400 (1504902)	1504902..1506116	-	1215	WP_001326689.1	BenE family transporter YdcO	-
LAJ62_RS07405 (1506169)	1506169..1506705	+	537	WP_000429155.1	DNA-binding transcriptional regulator SutR	-
LAJ62_RS07410 (1506778)	1506778..1508739	+	1962	WP_001303492.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
LAJ62_RS07415 (1508831)	1508831..1509061	-	231	WP_000494244.1	YncJ family protein	-
LAJ62_RS07420 (1509283)	1509283..1509459	+	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
LAJ62_RS07425 (1509505)	1509505..1509921	+	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
LAJ62_RS07430 (1510000)	1510000..1511406	+	1407	WP_000760626.1	PLP-dependent aminotransferase family protein	-
LAJ62_RS07435 (1511651)	1511651..1512796	+	1146	WP_000047424.1	ABC transporter substrate-binding protein	-
LAJ62_RS07440 (1512814)	1512814..1513827	+	1014	WP_000220396.1	ABC transporter ATP-binding protein	-
LAJ62_RS07445 (1513828)	1513828..1514769	+	942	WP_001251304.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T216369 WP_000813794.1 NZ_CP083410:1509283-1509459 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T216369 NZ_CP113029:1202179-1202541 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAGTTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCACCAGAAAAATTAG

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT216369 WP_001270286.1 NZ_CP083410:1509505-1509921 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT216369 NZ_CP113029:1202012-1202182 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Toxin

• PDB: 6HPB