Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 480090..480708 Replicon chromosome
Accession NZ_CP083410
Organism Escherichia coli strain RP437

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag LAJ62_RS02365 Protein ID WP_001291435.1
Coordinates 480090..480308 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag LAJ62_RS02370 Protein ID WP_000344800.1
Coordinates 480334..480708 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LAJ62_RS02330 (475379) 475379..475951 + 573 WP_000779842.1 YbaY family lipoprotein -
LAJ62_RS02335 (475982) 475982..476293 - 312 WP_000409911.1 MGMT family protein -
LAJ62_RS02345 (476672) 476672..477025 + 354 WP_000878140.1 DUF1428 domain-containing protein -
LAJ62_RS02350 (477067) 477067..478617 - 1551 WP_001310610.1 cyclic-guanylate-specific phosphodiesterase PdeB -
LAJ62_RS02355 (478781) 478781..479251 - 471 WP_000136192.1 YlaC family protein -
LAJ62_RS02360 (479367) 479367..479918 - 552 WP_000102564.1 maltose O-acetyltransferase -
LAJ62_RS02365 (480090) 480090..480308 - 219 WP_001291435.1 HHA domain-containing protein Toxin
LAJ62_RS02370 (480334) 480334..480708 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
LAJ62_RS02375 (481254) 481254..484403 - 3150 WP_001132469.1 efflux RND transporter permease AcrB -
LAJ62_RS02380 (484426) 484426..485619 - 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T216358 WP_001291435.1 NZ_CP083410:c480308-480090 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T216358 NZ_CP113027:1445642-1445794 [Staphylococcus aureus]
ATGTCTAAAGACAAAGATCCAAAATTAAATTATCATGAAGAAGAAAACAGTATGGTAACGGATTTTGAAGATTTAAAAGA
ATTAGGTAAAGAAATGGAACAAATTTCTGATCAAAATGATCAAGAAAAGAATTCTGAAGAAGACAGTCAGTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT216358 WP_000344800.1 NZ_CP083410:c480708-480334 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT216358 NZ_CP113027:1445018-1445617 [Staphylococcus aureus]
ATGGCAATGAACTTTAAAGTCTTTGACAATAGTCAACTTGTAGCAGAATATGCTGCTGATATTATTAGAAAGCAATTTAA
CAATAATCCTACTACAATTGCAGGTTTTCATTTAGATACTGATCAAGCGCCAGTACTAGATGAATTAAAGAAAAATATTG
AAAAGCATGCTGTTGATTTTAGCCAAATAAATATTTTAGATTATGACGATAAAAAATCATATTTCGAAGCGTTAGGTGTA
CCAGCAGGTCAAGTTTATCCAATTGCTTATGAAAAAGATGCAATCGAATTAATCGCTGATAAGATTAAAACTAAAGAAAA
TAAAGGGAAATTAACATTACAAGTTGTTTCTATTGATGAGCAAGGTAAGTTAAATGTTAGTATTCGTCAAGGACTAATGG
AAGCAAGAGAAATTTTCATAGTAGTGACTGGTGCTAATAAACGAGATGTAGTTGAAAAATTATATCAAGAAAATGGTAAA
ACAAGCTTCGAACCAGCCGATTTAAAAGCACATAGAATGGTAAATGTTATTCTTGATAAAGAAGCGGCTGCAGGTTTACC
TGAAGATGTTAAAGCTTACTTTACGTCACGCTTTGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References