Detailed information of TA system TA216357

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yafW-ykfI/CbtA-CbeA
• Location: 263328..264007
• Replicon: chromosome
• Accession: NZ_CP083410
• Organism: Escherichia coli strain RP437

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: P77692
• Locus tag: LAJ62_RS01255
• Protein ID: WP_000854672.1
• Coordinates: 263328..263669 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yafW
• Uniprot ID: Q47684
• Locus tag: LAJ62_RS01260
• Protein ID: WP_000070395.1
• Coordinates: 263690..264007 (-)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LAJ62_RS01230 (258677)	258677..259006	+	330	Protein_238	sigma factor-binding protein Crl	-
LAJ62_RS01235 (259045)	259045..260100	-	1056	WP_000749863.1	phosphoporin PhoE	-
LAJ62_RS01240 (260388)	260388..261491	+	1104	WP_001285288.1	glutamate 5-kinase	-
LAJ62_RS01245 (261503)	261503..262756	+	1254	WP_000893278.1	glutamate-5-semialdehyde dehydrogenase	-
LAJ62_RS01255 (263328)	263328..263669	-	342	WP_000854672.1	type IV toxin-antitoxin system toxin YkfI	Toxin
LAJ62_RS01260 (263690)	263690..264007	-	318	WP_000070395.1	type IV toxin-antitoxin system antitoxin YafW	Antitoxin
LAJ62_RS01265 (264026)	264026..264247	-	222	WP_000691994.1	DUF987 domain-containing protein	-
LAJ62_RS01270 (264256)	264256..264732	-	477	WP_000811693.1	RadC family protein	-
LAJ62_RS01275 (264748)	264748..265206	-	459	WP_000211838.1	antirestriction protein	-
LAJ62_RS01280 (265304)	265304..265543	-	240	WP_000194654.1	DUF905 family protein	-
LAJ62_RS01285 (265620)	265620..266087	-	468	WP_001547765.1	protein YkfB	-
LAJ62_RS01290 (266110)	266110..266553	-	444	WP_000824223.1	lipoprotein YafY	-
LAJ62_RS01295 (266553)	266553..266729	-	177	WP_001285112.1	hypothetical protein	-
LAJ62_RS01300 (266776)	266776..266967	-	192	Protein_251	DeoR family transcriptional regulator	-
LAJ62_RS01305 (267184)	267184..268005	-	822	WP_000197389.1	DUF932 domain-containing protein	-
LAJ62_RS01310 (268097)	268097..268960	-	864	WP_001065553.1	GTPase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (6-104)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12905.01 Da
• Isoelectric Point: 9.6543

>T216357 WP_000854672.1 NZ_CP083410:c263669-263328 [Escherichia coli]
MKTLPAITQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAADILRARQATGLLRQSRNNVVR

Nucleotide

• Length: 342 bp

>T216357 NZ_CP113027:1293227-1293589 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAATTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCATCAGAAAAATTAG

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11939.59 Da
• Isoelectric Point: 6.7391

>AT216357 WP_000070395.1 NZ_CP083410:c264007-263690 [Escherichia coli]
MSNPTRGLQREITLRLGARLVQEGNRLHYLADRASITGKFSDIECRKLDETFPHFILQMESMLTTGELSPHHAHCVTLYH
NDLTCEADTLGSCGYVYIAIYPTQR

Nucleotide

• Length: 318 bp

>AT216357 NZ_CP113027:1293060-1293230 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Toxin

• AlphaFold DB: P77692

Antitoxin

• AlphaFold DB: Q47684