Detailed information of TA system TA216354

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): unclassified/Polyketide_cyc2(toxin)
• Location: 2961690..2962329
• Replicon: chromosome
• Accession: NZ_CP083405
• Organism: Mycobacterium lepromatosis strain FJ924

Toxin (Protein)

• Gene name: novel[antitoxin]
• Uniprot ID: -
• Locus tag: MLPF_RS14020
• Protein ID: WP_045843605.1
• Coordinates: 2961883..2962329 (+)
• Length: 149 a.a.

Antitoxin (Protein)

• Gene name: novel[toxin]
• Uniprot ID: A0A0F4EP48
• Locus tag: MLPF_RS14015
• Protein ID: WP_045843606.1
• Coordinates: 2961690..2961878 (+)
• Length: 63 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MLPF_RS13985 (MLPF_3083)	2957297..2958354	+	1058	Protein_2813	MBL fold metallo-hydrolase	-
MLPF_RS13990	2958664..2958939	+	276	WP_236685145.1	hypothetical protein	-
MLPF_RS13995	2959469..2959609	+	141	WP_237756019.1	hypothetical protein	-
MLPF_RS14000	2960201..2960620	+	420	WP_237817215.1	hypothetical protein	-
MLPF_RS14005	2960752..2960976	+	225	WP_237756018.1	hypothetical protein	-
MLPF_RS14010	2961309..2961596	+	288	WP_236685140.1	hypothetical protein	-
MLPF_RS14015 (MLPF_3086)	2961690..2961878	+	189	WP_045843606.1	antitoxin	Antitoxin
MLPF_RS14020 (MLPF_3087)	2961883..2962329	+	447	WP_045843605.1	SRPBCC family protein	Toxin
MLPF_RS14025 (MLPF_3089)	2963292..2963726	+	435	WP_045843604.1	hypothetical protein	-
MLPF_RS14030 (MLPF_3090)	2963731..2964215	-	485	Protein_2822	VOC family protein	-
MLPF_RS14035 (MLPF_3091)	2964223..2965696	-	1474	Protein_2823	carotenoid oxygenase family protein	-
MLPF_RS14040	2965827..2966105	-	279	WP_156147226.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polyketide cyclase/dehydrase (1-141)

Antitoxin

• Antitoxin Rv0909/MT0933 (4-52)

Sequences

Toxin

Protein

• Length: 149 a.a.
• Molecular weight: 16205.90 Da
• Isoelectric Point: 7.8559

>T216354 WP_045843605.1 NZ_CP083405:2961883-2962329 [Mycobacterium lepromatosis]
MAKLTGSIDVPLPPDEAWMCASDLSRFDEWLTIHRVWRSKLPEVVEKGTVIESYVEVKGMSNRIKWTVVRYKAPEAMTLN
GDGVGGVKVKMIAKVAPKDDGSVVSFDVHLGGPALCGPIGLIVAVALRSDIRESLQNFVAVFSRPERV

Nucleotide

• Length: 447 bp

>T216354 NZ_CP113027:906302-906568 [Staphylococcus aureus]
ATGGCTAGGTTAAATATTACGTTTTCGCCTCAAGCCTTTGAAGATTATAAGTATTTTCAGCAGAACGATAAAAAAATGGT
GAAGAAGATTAATGAGTTACTTAAAAGTATTGACAGAAATGGTGCATTGAAAGGTATAGGTAAGCCTGAAAAGTTAAAAT
CGAATCTGACTGGTTATTATAGTAGACGTATCAATCACGAACATAGATTGGTCTATACAGTAGATGACAATCATATAAAA
ATAGCATCATGTAAATACCATTATTAA

Antitoxin

Protein

• Length: 63 a.a.
• Molecular weight: 6818.62 Da
• Isoelectric Point: 4.5105

>AT216354 WP_045843606.1 NZ_CP083405:2961690-2961878 [Mycobacterium lepromatosis]
MGILDKAKDLLVQNADKVKTVLDRVGEFVDEKTKGQYIDAIYKVAEEAKKAASSDTSSDQQD

Nucleotide

• Length: 189 bp

>AT216354 NZ_CP113027:906051-906302 [Staphylococcus aureus]
ATGATTATTAAAAATTATTCATACGCTCGACAGAATTTAAAGGCACTTATGACAAAAGTAAATGATGATAGTGATATGGT
AACTGTAACCTCTACTGATGATAAAAACGTAGTAATCATGTCAGAATCAGATTATAACTCCATGATGGAAACACTTTACC
TCCAACAGAATCCAAATAATGCTGAACACTTAGCTCAATCAATTGCAGATCTAGAACGTGGAAAAACTATAACGAAAGAT
ATAGATGTATAA

Structures

Antitoxin

• AlphaFold DB: A0A0F4EP48