Detailed information of TA system TA021635

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4022100..4022932
• Replicon: chromosome
• Accession: NC_008253
• Organism: Escherichia coli 536

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: Q8GA15
• Locus tag: ECP_RS19580
• Protein ID: WP_000854725.1
• Coordinates: 4022558..4022932 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A454A9R1
• Locus tag: ECP_RS19575
• Protein ID: WP_011579284.1
• Coordinates: 4022100..4022468 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECP_RS19540	4018022..4018702	+	681	WP_001282920.1	WYL domain-containing protein	-
ECP_RS19545	4018850..4019527	+	678	WP_001097308.1	hypothetical protein	-
ECP_RS19550	4019533..4019766	+	234	WP_001278287.1	DUF905 family protein	-
ECP_RS19555	4019856..4020674	+	819	WP_001175163.1	DUF945 domain-containing protein	-
ECP_RS19560	4020766..4021251	+	486	WP_000206652.1	antirestriction protein	-
ECP_RS19565	4021266..4021742	+	477	WP_001186726.1	RadC family protein	-
ECP_RS19570	4021805..4022026	+	222	WP_000692323.1	DUF987 domain-containing protein	-
ECP_RS19575	4022100..4022468	+	369	WP_011579284.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
ECP_RS19580	4022558..4022932	+	375	WP_000854725.1	TA system toxin CbtA family protein	Toxin
ECP_RS19585	4022929..4023408	+	480	WP_000988603.1	hypothetical protein	-
ECP_RS19590	4023420..4023617	+	198	WP_000839274.1	DUF957 domain-containing protein	-
ECP_RS19595	4023701..4024546	+	846	WP_001280468.1	DUF4942 domain-containing protein	-
ECP_RS19605	4025139..4025637	+	499	Protein_3728	hypothetical protein	-
ECP_RS19610	4025804..4026727	+	924	WP_001351210.1	carboxylate/amino acid/amine transporter	-
ECP_RS19615	4026731..4027549	-	819	WP_000779409.1	lipoprotein NlpA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13996.03 Da
• Isoelectric Point: 7.8019

>T21635 WP_000854725.1 NC_008253:4022558-4022932 [Escherichia coli 536]
MKTLPDTHVREASRCPSPVTIWQTLLIHLLGQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Nucleotide

• Length: 375 bp

>T21635 NC_008253:4022558-4022932 [Escherichia coli 536]
ATGAAAACATTACCCGATACACACGTACGGGAGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAT
CCATCTGCTGGGCCAGCACTATGGCCTCACACTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACTCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13505.40 Da
• Isoelectric Point: 6.0567

>AT21635 WP_011579284.1 NC_008253:4022100-4022468 [Escherichia coli 536]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLVLMLTSG
ELSPRIQHTVTLYAKGLTCEADTLGSCGYVYLAFYPTSETKK

Nucleotide

• Length: 369 bp

>AT21635 NC_008253:4022100-4022468 [Escherichia coli 536]
GTGTCAGACACACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTACCCTGCACCGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGTGCTCATGCTCACCAGTGGT
GAACTCAGCCCCCGCATACAGCATACCGTCACGCTGTATGCGAAAGGACTGACCTGCGAAGCCGACACCCTCGGCAGTTG
TGGTTACGTTTATCTGGCTTTTTATCCAACATCCGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A454A9L2

Antitoxin

• AlphaFold DB: A0A454A9R1